Cytotoxic,antitumour-promoting and inhibition of protein denaturation effects of flavonoids,isolated from Potentilla evestita Th. Wolf |
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| Authors: | Abdur Rauf Rehan Khan Harukuni Tokuda |
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| Institution: | 1. Institute of Chemical Sciences, University of Peshawar, Peshawar 25120, KPK, Pakistan;2. HEJ Research Institute, Institute of Chemical and Biological Sciences, Karachi University, Karachi, Pakistan;3. Department of Complementary and Alternative Medicine, Clinical R&4. D, Graduate School of Medical Science, Kanazawa University, 13-1 Takara-machi, Kanazawa 920-8640, Japan |
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| Abstract: | This study was designed to evaluate the isolated flavonoids (chrysin 1, and umbelliferone 2) from Potentilla evestita for cytotoxic, antitumour-promoting and inhibition of protein denaturation activities. The results showed marked cytotoxic effect of compounds 1 and 2 in brine shrimp cytotoxic assay at various concentrations with LD50 of 34.5 and 31.8 mg/mL, respectively. In Epstein–Barr-virus early antigen activation assay, both compounds 1 and 2 illustrated significant antitumour-promoting effect with IC50 values of 462 and 308 mol ratio/32 pmol TPA, respectively. The cytotoxic and antitumour-promoting effects of compounds were strongly supported by inhibition of protein denaturation activity with IC50 of 119 and 112 μg/mL, respectively. In conclusion, both compounds possess strong cytotoxic, antitumour-promoting and inhibition of protein denaturation activities. |
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| Keywords: | Potentilla evestita chrysin and umbelliferone cytotoxic antitumour-promoter and inhibition of protein denaturation |
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