Effect of bioclimatic area on the composition and bioactivity of Tunisian Rosmarinus officinalis essential oils |
| |
Authors: | Mariem Ben Jemia Alessandro Pugliese Francesco Menichini Felice Senatore Maurizio Bruno |
| |
Institution: | 1. Laboratoire des Plantes Extremophiles – Biotechnologic Center Borj-Cedria Technopark, B.P. 901, 2050 Hammam-Lif, Tunisia;2. Department of Pharmacy, Health Sciences and Nutrition, University of Calabria, I-87036 Rende (CS), Italy;3. Department of Chemistry of Natural Products, University of Naples “Federico II”, Via D. Montesano, 49-80131 Naples, Italy;4. Department of Pharmacy, University of Naples “Federico II”, Via D. Montesano, 49-80131 Naples, Italy;5. Department STEBICEF, University of Palermo, Viale delle Scienze, Parco d'Orleans II, 90128 Palermo, Italy |
| |
Abstract: | The chemical composition of eight Tunisian Rosmarinus officinalis L. populations (A–H) from different bioclimatic areas has been examined by gas chromatography (GC) and GC-mass spectrometry. The essential oils are characterised by high amounts of oxygenated monoterpenes (58.2–71.7%) followed by monoterpene hydrocabons (15.1–26.7%). 1,8-Cineole, camphor, α-pinene and borneol are the main representative components. The antioxidant activity was investigated by 2,2-diphenyl-1-picrylhydrazyl radical (DPPH), ferric reducing ability power assay and β-carotene bleaching test. Samples showed antiradical activity by inhibiting DPPH radical with IC50 values ranging from 375.3 to 592.8 μg mL? 1 for samples F and A, respectively. Sample A also showed the most promising activity in β-carotene bleaching test (IC50 of 31.9 μg mL? 1). The essential oils were also screened for acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibitory activity. Sample G showed the highest activity against AChE (IC50 of 64.7 μg mL? 1) while sample D (IC50 of 29.5 μg mL? 1) exhibited the most potent activity against BChE. |
| |
Keywords: | Rosmarinus officinalis essential oil GC-MS analysis antioxidant properties cholinesterase inhibitory activity |
|
|