A new tetracyclic saponin from Astragalus glycyphyllos L. and its neuroprotective and hMAO-B inhibiting activity |
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| Authors: | Aleksandar Shkondrov Franz Bucar Olaf Kunert Magdalena Kondeva-Burdina Iliana Ionkova |
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| Institution: | 1. Department of Pharmacognosy, Faculty of Pharmacy, Medical University of Sofia, Sofia, Bulgaria;2. Department of Pharmacognosy, Institute of Pharmaceutical Sciences, University of Graz, Graz, Austria;3. Department of Pharmaceutical Chemistry, Institute of Pharmaceutical Sciences, University of Graz, Graz, Austria;4. Laboratory of Drug Metabolism and Drug Toxicity, Department of Pharmacology, Pharmacotherapy and Toxicology, Faculty of Pharmacy, Medical University of Sofia, Sofia, Bulgaria |
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| Abstract: | AbstractA new tetracyclic saponin, 17(R),20(R)-3β,6α,16β-trihydroxycycloartanyl-23-carboxylic acid 16-lactone 3-O-β-D-glucopyranoside (1) together with one known flavonoid, camelliaside A (2) were isolated from the aerial parts of Astragalus glycyphyllos L. Their structures were determined by chemical, HRESIMS and NMR methods. On 6-hydroxydopamine in vitro model on isolated rat brain synaptosomes, compounds 1–2 had statistically significant neuroprotective activity similar to that of Silibinin, tested at 100?μM. Saponin 1 possessed the most prominent neuroprotective and antioxidant effects in this in vitro model. On human recombinant monoamine oxidase type B enzyme compound 1 displayed strong inhibiting activity, compared to Selegiline (1?μM). It could be concluded that the new epoxycycloartane saponin 1 could be a promising leading structure in respect of neurodegenerative diseases. |
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| Keywords: | Astragalus glycyphyllos saponins flavonoids neuroprotective activity synaptosomes |
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