Cytotoxic naphthoquinones from Arnebia densiflora (Nordm.) Ledeb and determining new apoptosis inducers |
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| Authors: | Merve Yuzbasioglu Baran Zuhal Guvenalp Iclal Saracoglu Cavit Kazaz Bekir Salih L Omur Demirezer |
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| Institution: | 1. Department of Pharmacognosy, Faculty of Pharmacy, Hacettepe University, Ankara, Turkey;2. Department of Pharmacognosy, Faculty of Pharmacy, Ataturk University, Erzurum, Turkey;3. Department of Chemistry, Faculty of Sciences, Ataturk University, Erzurum, Turkey;4. Department of Chemistry, Faculty of Science, Hacettepe University, Ankara, Turkey |
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| Abstract: | AbstractIn this study, phytochemical composition of Arnebia densiflora (AD) was determined and cytotoxic effects of the n-hexane extract and compounds isolated from this species on various cell lines were investigated. By means of serial chromatographic studies, 6 naphthoquinone derivatives were yielded, which are isovalerylalkannin, α-methyl-n-butyl alkannin, acetylalkannin, β-acetoxy isovalerylalkannin, alkannin and a new compound: 4-hydroxy 4-methyl valeryl alkannin. Structures of the isolated compounds were elucidated using UV, IR, 1D-2D NMR, MS and CD methods. Cytotoxic effects of the extract and isolated alkannins were investigated on L929, HeLa, HEp-2 cells. AD and the isolated compounds demonstrated moderate to strong cytotoxic effects (IC50 range: 4.92-172.35?µg/ml). The results of DNA fragmentation and caspase-3 activity studies on HeLa cells exhibited that AD and the naphthoquinones isolated from it caused cytotoxicity through induction of apoptosis. | |
| Keywords: | Alkannin cytotoxicity caspase apoptosis Arnebia densiflora Boraginaceae |
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