Antidiarrhoeic effect and dereplication of the aqueous extract of Annona crassiflora (Annonaceae) |
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| Authors: | Camila Rodrigues Ferraz Denise Brentan Silva Ligia Carolina da Silva Prado Hudson Armando Nunes Canabrava |
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| Institution: | 1. Department of Pharmacology, Institute of Biomedical Sciences, Federal University of Uberlandia, Minas Gerais, Brazil;2. Faculty of Pharmaceutical Science at Ribeir?o Preto, Núcleo de Pesquisas em Produtos Naturais e Sintéticos (NPPNS), University of S?o Paulo (FCFRP-USP), S?o Paulo, Brazil;3. Laboratório de Produtos Naturais e Espectrometria de Massas (LAPNEM), Federal University of Mato Grosso do Sul (UFMS), Mato Grosso do Sul, Brazil |
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| Abstract: | We investigated the antidiarrhoeic effect of the aqueous extract of Annona crassiflora leaves (AEAC). The AEAC decreased the diarrhoeic stools and enteropooling induced by castor oil, without altering total faecal output; moreover, the distance travelled by charcoal meal in the intestine was increased. Twenty-eight compounds were identified by LC-DAD-MS in the AEAC, including flavonoids, alkaloids and proanthocyanidins. In addition, two oligomeric series of condensed tannins of up to nine flavan-3-ol units were characterised by MALDI-MS. These data suggest that the antidiarrhoeic effect of the AEAC is related to its ability to inhibit intestinal secretion and/or to increase intestinal absorption. Moreover, the prokinetic effect of AEAC, together with its inhibitory effect on enteropooling induced by castor oil, explains why this extract decreased diarrhoeic faeces without altering the total faecal output. All these effects are in agreement with the pharmacological activity reported in the literature for many of the secondary metabolites identified. |
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| Keywords: | Annona crassiflora antidiarrhoeic dereplication castor oil |
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