Alkaloids from Juglans Mandshurica maxim induce distinctive cell death in hepatocellular carcinoma cells |
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| Authors: | Li-Li Lou Zhuo-Yang Cheng Rui Guo |
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| Institution: | 1. School of Traditional Chinese Materia Medica, Key Laboratory of Structure-Based Drug Design &2. Discovery (Ministry of Education), Shenyang Pharmaceutical University , Shenyang, People’s Republic of China |
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| Abstract: | The aim of this work was to further investigate the anticancer potential of Juglans mandshurica Maxim, including the separation of active constituents and their anti-proliferative effects with underlying mechanism of action. Five alkaloids (1–5) were isolated from the bark of J. mandshurica. Among them, 1 showed the highest cytotoxic activities against Hep3B and HepG2 cells with an IC50 values of 61.80 and 56.24 μM, respectively. Therefore, the cellular mechanism involved 1 was subsequently studied. Our results showed that 1 markedly caused apoptosis and autophagy, but without cell cycle arrest in HepG2 cells. Interestingly, only autophagic cell death was induced in 1-treated Hep3B cells. It is concluded that the isolated alkaloids exerted a certain anti-hepatoma potential, and our results may provide a basis for the further investigation of the alkaloids extracted from J. mandshurica. |
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| Keywords: | Juglans mandshurica Alkaloids Hepatocellular carcinoma Apoptosis Autophagy |
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