Hilic MS/MS determination of amino acids in herbs of Fumaria schleicheri L., Ocimum basilicum L., and leaves of Corylus avellana L. |
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| Authors: | Yuliya Prokopenko Valdas Jakštas Vaidotas Žvikas Victoriya Georgiyants Liudas Ivanauskas |
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| Institution: | 1. Department of Quality, Standardisation and Certification of Drugs, National University of Pharmacy , Kharkiv, Ukraine;2. Faculty of Pharmacy, Medical Academy, Department of Pharmacognosy, Lithuanian University of Health Sciences , Kaunas, Lithuania;3. Department of Pharmaceutical Chemistry, National University of Pharmacy , Kharkiv, Ukraine;4. Faculty of Pharmacy, Medical Academy, Department of Analytical and Toxicological Chemistry, Lithuanian University of Health Sciences , Kaunas, Lithuania |
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| Abstract: | The aim of research was to study the content of amino acids using in extracts of Fumaria schleicheri L., Ocimum basilicum L., and Corylus avellana L. by HILIC MS/MS method. Separation of amino acids in the samples was carried out with Acquity H-class UPLC system (Waters, Milford, USA) equipped with SeQuant ZIC-Hilic collumn (2.1 × 150 mm, 3.5 μm) (Merck Millipore, Darmstadt, Germany). The MS/MS fragment ion chromatograms of the test solutions established the presence of 19 amino acids. The obtained results have shown that O. basilicum L. characterized the highest concentrations of different neurogenic amino acids (128.1 mg/kg), comparing with F. schleicheri L. and C. avellana L. (57.72 and 52.91 mg/kg, respectively). |
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| Keywords: | Amino acids Fumaria schleicheri L Ocimum basilicum L Corylus avellana L HILIC MS/MS |
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