Saponin-rich fraction from Agave sisalana: effect against malignant astrocytic cells and its chemical characterisation by ESI-MS/MS |
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| Authors: | João Victor A de Oliveira Jener D G dos Santos Deivison S Argolo Victor Diogenes A da Silva Gisele D da Silva |
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| Institution: | 1. Laboratório de Neuroquímica e Biologia Celular, Departamento de Bioquímica e Biofísica, Instituto de Ciências da Saúde, Universidade Federal da Bahia, Salvador, Brazil;2. Departamento de Saúde, Universidade Estadual de Feira de Santana, Feira de Santana, Brazil;3. Departamento de Patologias e Clínicas, Escola de Medicina Veterinária, Universidade Federal da Bahia, Salvador, Brazil |
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| Abstract: | Astrocytic tumour cells derived from human (GL-15) and rat (C6) gliomas, as well as non-tumoural astrocytic cells, were exposed to the saponin-rich fraction (SF) from Agave sisalana waste and the cytotoxic effects were evaluated. Cytotoxicity assays revealed a reduction of cell viability that was more intensive in glioma than in non-tumoural cells. The SF induced morphological changes in C6 cells. They were characterised by cytoplasmic vacuole formation associated with increase in the formation of acidic lysosomes. The SF was subjected to purification on Sephadex LH-20, which characterised three probable steroidal saponins (sisalins) by electrospray ionisation mass spectrometry multistage (ESI-MSn). Sisalins from sisal may be responsible for the cytotoxicity, which involves cytoplasmatic vacuole formation and selective action for glioma cells. |
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| Keywords: | Agave sisalana cytotoxicity glioma ESI/MS saponin |
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