A simple method for extracting both active oily and water soluble extract (WSE) from Nigella sativa (L.) seeds using a single solvent system |
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| Authors: | ZM Babar Wan Mohd Azizi Solachuddin JA Ichwan Qamar Uddin Ahmed Abul Kalam Azad Imranul Mawa |
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| Institution: | 1. Basic Medical Sciences, Faculty of Pharmacy, International Islamic University Malaysia, Kuantan, Malaysia;2. Basic Medical Sciences, International Islamic University Malaysia, Kuantan, Malaysia;3. Faculty of Dentistry, International Islamic University Malaysia, Kuantan, Malaysia;4. Pharmaceutical Chemistry, International Islamic University Malaysia, Kuantan, Malaysia;5. Pharmaceutical Technology, International Islamic University Malaysia, Kuantan, Malaysia;6. Microbiology, University of Science and Technology Chittagong, Chittagong, Bangladesh |
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| Abstract: | The current study provides a way of extraction for both active NSO and WSE from Nigella sativa seeds using 98% methanol. About 1?kg of ground seeds was macerated by 1:2.5 w/v (g/mL) for 72?hours. After rotary evaporation and 7 days of continuous drying and chilling at 50 and 4?°C, NSO and WSE were obtained at the same instant. Solubility tests of 24 solvents and 11 thin layer chromatographic analyses while 2, 2-diphenyl-1-picrylhydrazyl free radical scavenging assay of NSO (73.66) , WSE (33.32) and NSO?+?WSE (78.22) against ascorbic acid (IC50?=?4.28?mg/mL) was performed. WSE was found to be highly soluble in water and 5% NaOH exhibiting the same Rf value of 0.95 for EtOH:DMSO (9:1) against the honey. WSE has revealed more than twofold higher anti-oxidant activity than others. Formulation of WSE with Tualang honey may provide better targeted hydrophilic drug delivery systems. |
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| Keywords: | N sativa seeds extraction solubility thin layer chromatography antioxidant NSO WSE |
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