Electrochemical Detection of Human Interleukin-15 using a Graphene Oxide-Modified Screen-Printed Carbon Electrode |
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Authors: | Poachanee Norfun Nuttee Suree Nawee Kungwan Winita Punyodom Jaroon Jakmunee |
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Institution: | Department of Chemistry and Center of Excellence for Innovation in Chemistry (PERCH-CIC), Faculty of Science, Chiang Mai University, Chiang Mai, Thailand |
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Abstract: | Biofunctionalizing a simple and disposable graphene oxide-modified screen-printed carbon electrode with anti-interleukin-15 antibodies has been successfully demonstrated for the first time for the label-free electrochemical detection of interleukin-15, a biomarker of early HIV infection. To improve the electrochemical reactivity and introduce carboxylic groups on the surface of screen-printed carbon electrode, high-quality graphene oxide was used for the modification of screen-printed carbon electrode. With simple modification of the screen-printed carbon electrode, the device exhibited satisfactory sensitivity, selectivity, stability, reproducibility, and regenerability. The immunosensor provided a detection limit of 3.51?ng?mL?1 and a sensitivity of 0.5655?µA cm?2?mL?ng?1. The simply constructed immunosensor thus rendered promising device for immunoreactions on the surface of the electrode. |
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Keywords: | Biomarkers early HIV-1 infection electrochemical detection graphene oxide immunosensor interleukin-15 screen-printed carbon electrode |
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