Determination of Anagliptin in Serum by Mixed-Hemimicelle Magnetic Solid-Phase Extraction with Surfactant-Coated Iron(II,III) Nanocomposites and High-Performance Liquid Chromatography

Magnetic solid-phase extraction based on the adsorption of sodium dodecyl sulfate on the surface of Fe₃O₄ nanoparticles was used to isolate the new hypoglycemic drug anagliptin in human and mouse serum before determination by high-performance liquid chromatography. The magnetic adsorbent was characterized by transmission electron microscopy, Fourier transform infrared spectroscopy, and the zeta potential. The factors affecting the extraction performance such as the type of surfactant, the amount of adsorbent and sodium dodecyl sulfate, pH of solution, time and temperature of adsorption and elution, and eluent type were examined. Under the optimized conditions, the adsorbent could be reused for six times and the efficiency of extraction or elution was over 95.0%. The calibration curve was linear in the range of 0.050–4.00?µg?mL^?1, with detection limits of 0.021 and 0.023?µg?mL^?1 for human and mouse serum, respectively. The recovery values of 92.0–99.1% (human serum) or 94.8–105.7% (mouse serum) illustrated the accuracy of the proposed method. Moreover, it may be the first time that this extraction method has been used to determine anagliptin in biological liquids.