Electrochemical Regeneration of NAD Covalently Bound to Liver Alcohol Dehydrogenase

Abstract

An enzyme-coenzyme complex was immobilized on the surface of a glassy carbon electrode and investigated with cyclic voltammetry in ethanol-containing buffers. The complex consists of an Liver Alcohol Dehydrogenase molecule to which an NAD-analogue is covalently attached via its straight six-carbon, spacer. One cycle was observed but repeated recycling could not be carried out. presumably due to catalytic decomposition of the coenzyme at the electrode surface.