Utilisation de la Vanilline et de Son Oxime Comme Etalon Interne Dans les Dosages des Acide-Phenols par Chromatographie Liquide: I. Application au Dosage de l'Acide Homovanillique Urinaire

Abstract

An HPLC method of standardization for acid-phenol (especially HVA) determination in urine is proposed. Urine spiked with vanillal is extracted with ethyl acetate. Exact area of the vanillal chromatographic peak is measured by difference beetwen two chromatograms : one of the urinary extract and one of the same extract submitted to oximation reaction. The method allows reliable internal standardization even when various urinary compounds are superposed to vanillal on the chromatogram. Correlation with CPG for HVA determination is excellent.