Abstract: | Abstract Simultaneous estimation of active ingredients in multicomponent pharmaceutical products normally requires the use of separation techniques, such as HPLC, HPTLC, or GC, followed by their quantitation. Presented here are two spectrophotometric methods that do not require prior separation for simultaneous estimation of three drugs—pioglitazone HCl, metformin HCl, and glibenclamid—in a tablet formulation. Shimadzu UV 1700, capable of multicomponent analysis, was used for quantitation. In method I, absorbance of the sample solution was measured at 285 nm and 300 nm for the estimation of pioglitazone HCl and glibenclaimide, and at 237 nm for estimation of metformin hydrochloride, respectively. Method II is based on a multiwavelength spectroscopic method. Recording the absorbances of standard solutions at 237 nm, 268 nm, 280 nm, and 300 nm were processed by means of statistical calculations and results of the sample solution were obtained. All three drugs obey Beer's law in the concentration ranges used for the methods. The result of the analysis for both methods were tested and validated for various parameters according to ICH guidelines. The utility of the developed methods has been demonstrated by an analysis of commercial formulation containing all the three drugs. |