Preparation of Cerium Dioxide Functionalized Magnetic Layered Double Hydroxides for High-efficiency Phosphopeptide Enrichment |
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Authors: | JIANG Dandan QI Ruixue LYU Siqi WANG Wei LIU Jinghai JIA Qiong |
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Institution: | 1. College of Chemistry and Materials Science, Inner Mongolia Key Laboratory of Carbon Nanomaterials, Inner Mongolia Minzu University, Tongliao 028000, P. R. China;2. College of Chemistry, Jilin University, Changchun 130012, P. R. China |
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Abstract: | In this work, we prepared a material with magnetic nanoparticles (Fe3O4) as core, layered double hydroxides(LDHs) as affinity shell, and cerium dioxide(CeO2) as functional molecules(denoted as Fe3O4@LDH-CeO2). On the basis of combined immobilized metal ion affinity chromatography(IMAC) and metal oxide affinity chromatography(MOAC), Fe3O4@LDH-CeO2 was used to enrich phosphopeptides with high efficiency. The material exhibited high selectivity(α-casein:β-casein:BSA=1:1:5000, mass ratio), high recovery(95.87%), and good reusability of 10 times adsorption- desorption experiments. The feasibility of Fe3O4@LDH-CeO2 was further investigated by extracting phosphopeptides from biological samples(nonfat milk, serum, saliva, and A549 cell lysate). |
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Keywords: | Layered double hydroxide Cerium dioxide Magnetic Phosphopeptide enrichment |
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