| CRISPR-Cas System for RNA Detection and Imaging |
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| 作者姓名： | CHEN Siyu WANG Rujia LEI Chunvang NIE Zhou |
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| 作者单位： | State Key Laboratory of Chemo/Biosensing and Chemometrics |
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| 基金项目： | Supported by the National Natural Science Foundation of China(Nos.21725503,21575038);the Young Top-notch Talent for Ten Thousand Talent Program of China. |
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| 摘 要： | The system of clustered regularly interspaced short palindromic repeats(CRISPR)and CRISPR-associated endonucleases(Cas)have been widely used in gene editing,disease treatment,molecular diagnosis and chromosome imaging.On account of the programmable target recognition of CRISPR-Cas system and the specific targeting function toward RNA of type Ⅵ class Ⅱ Cas proteins,CRISPR-Cas system has been deployed as RNA recognition and detection tools,exhibiting promising application potentials in the field of RNA detection and imaging.In this review,we summarize the latest research progresses as well as development prospects of CRISPR-Cas system in RNA diagnosis and live cell RNA imaging.
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| 关 键 词： | Clustered regularly interspaced short palindromic repeats-associated endonucleases(CRISPR-Cas) RNA detection RNA IMAGING COLLATERAL cleavage |
| 收稿时间： | 2019-10-29 |
CRISPR-Cas System for RNA Detection and Imaging |
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| CHEN Siyu,WANG Rujia,LEI Chunvang,NIE Zhou.CRISPR-Cas System for RNA Detection and Imaging[J].Chemical Research in Chinese University,2020,36(2):157-163. |
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| Authors: | CHEN Siyu WANG Rujia LEI Chunyang NIE Zhou |
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| Institution: | State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan Provincial Key Laboratory of Biomacromolecular Chemical Biology, Hunan University, Changsha 410082, P. R. China |
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| Abstract: | The system of clustered regularly interspaced short palindromic repeats(CRISPR) and CRISPR-associated endonucleases(Cas) have been widely used in gene editing, disease treatment, molecular diagnosis and chromosome imaging. On account of the programmable target recognition of CRISPR-Cas system and the specific targeting function toward RNA of type VI class II Cas proteins, CRISPR-Cas system has been deployed as RNA recognition and detection tools, exhibiting promising application potentials in the field of RNA detection and imaging. In this review, we summarize the latest research progresses as well as development prospects of CRISPR-Cas system in RNA diagnosis and live cell RNA imaging. |
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| Keywords: | Clustered regularly interspaced short palindromic repeats-associated endonucleases (CRISPR-Cas) RNA detection RNA imaging Collateral cleavage |
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