| Preparation and Kinetics of a Novel Glutathione Peroxidase Mimic- Selenium-modified Hyaluronic Acid |
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| 作者姓名： | CHEN Jia HONG Shui-sheng AN Yang JIANG Guang-zhi ZHANG Bo-xun FANG Xiang-yang LIU Lan-ying TENG Li-rong |
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| 作者单位： | College of life Science, Jilin University, Changchun 130021, P. R. China |
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| 摘 要： | A selenium-modified hyaluronic acid（HA） compound（SeHA） was synthesized as a mimic of glutathione peroxidase（GPX）. IR and NMR spectra of SeHA predict that the --CH2OH group of the N-acetyl-D-glucosamine in HA was modified by group --Sell. There are averagely 152 --Sell groups in one SeHA molecule. The GPX activities are 103.88, 32. 15 and 152. 71 U/μmol, respectively, when the substrates were H2O2, BuOOH and CuOOH. Ping-pong mechanism was observed in the steady-state kinetic studies of the reactive oxygen species（ROS） consuming reaction. Result of reactions of SeHA with free radical capturer 2,6-di-tert-butyl-4-methylphenol（BHT） shows that the catalysis is based on a non-free radical-mechanism and the SeHA can not be inhibited by iodoacetate, an enzyme inhibitor.
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| 关 键 词： | 谷胱甘肽过氧化物酶 硒修饰透明质酸 制备 酶动力学 |
| 收稿时间： | 13 April 2006. |
| 修稿时间： | 2006-04-13 |
Preparation and Kinetics of a Novel Glutathione Peroxidase Mimic—Selenium-modified Hyaluronic Acid |
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| CHEN Jia HONG Shui-sheng AN Yang JIANG Guang-zhi ZHANG Bo-xun FANG Xiang-yang LIU Lan-ying TENG Li-rong.Preparation and Kinetics of a Novel Glutathione Peroxidase Mimic- Selenium-modified Hyaluronic Acid[J].Chemical Research in Chinese University,2007,23(3):325-328. |
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| Authors: | Jia CHEN Shui-sheng HONG Yang AN Guang-zhi JIANG Bo-xun ZHANG Xiang-yang FANG Lan-ying LIU Li-rong TENG |
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| Institution: | aCollege of life Science, Jilin University, Changchun 130021, P. R. China |
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| Abstract: | A selenium-modified hyaluronic acid(HA) compound(SeHA) was synthesized as a mimic of glutathione peroxidase(GPX). IR and NMR spectra of SeHA predict that the -CH2OH group of the N-acetyl-D-glucosamine in HA was modified by group -SeH. There are averagely 152 -SeH groups in one SeHA molecule. The GPX activities are 103.88, 32.15 and 152.71 U/μmol, respectively, when the substrates were H2O2, BuOOH and CuOOH. Ping-pong mechanism was observed in the steady-state kinetic studies of the reactive oxygen species(ROS) consuming reaction. Result of reactions of SeHA with free radical capturer 2,6-di-tert-butyl-4-methylphenol(BHT) shows that the catalysis is based on a non-free radical-mechanism and the SeHA can not be inhibited by iodoacetate, an enzyme inhibitor. |
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| Keywords: | Selenium-modified hyaluronic acid GPX Preparation Kinetics |
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