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Inhibitory Effect of Anti-HER-2 Anti-CD3 Bi-specific Antibody on the Growth of Gastric Carcinoma
作者姓名:FANG Xue-dong  REN Hui  ZHANG Yan * and WANG Guan-jun . No. Affiliated Hospital  Jilin University  Changchun  P. R. China  . China-Japan Union Hospital  Jilin University  Changchun  P. R. China  . No. Affiliated Hospital  Jilin University  Changchun  P. R. China
作者单位:FANG Xue-dong 1,REN Hui 1,ZHANG Yan 2* and WANG Guan-jun 31. No.2 Affiliated Hospital,Jilin University,Changchun 130041,P. R. China; 2. China-Japan Union Hospital,Jilin University,Changchun 130033,P. R. China;3. No.1 Affiliated Hospital,Jilin University,Changchun 130022,P. R. China
摘    要:Introduction Gastriccancerisoneofthemostcommonlyen counteredmalignantdiseasesworldwide,especiallyin AsiaandAfrica1].Thecombinationofoperation,chemotherapyandradiotherapyisusedfortreatinggas triccarcinoma.However,the5yearsurvivalrateof patientsofgastricca…

关 键 词:Anti-HER-2×anti-CD3  bi-specific  antibody  HER-2/neu  Human  gastric  carcinoma  Nude  mice
文章编号:1005-9040(2006)-02-193-04
收稿时间:31 December 2005

Inhibitory Effect of Anti-HER-2 Anti-CD3 Bi-specific Antibody on the Growth of Gastric Carcinoma
FANG Xue-dong ,REN Hui ,ZHANG Yan * and WANG Guan-jun . No. Affiliated Hospital,Jilin University,Changchun ,P. R. China, . China-Japan Union Hospital,Jilin University,Changchun ,P. R. China,. No. Affiliated Hospital,Jilin University,Changchun ,P. R. China.Inhibitory Effect of Anti-HER-2 Anti-CD3 Bi-specific Antibody on the Growth of Gastric Carcinoma[J].Chemical Research in Chinese University,2006,22(2):193-196.
Authors:Xue-dong FANG  Hui REN  Yan ZHANG  Guan-jun WANG
Institution:aNo. 2 Affiliated Hospital, Jilin University, Changchun 130041, P. R. China;bChina-Japan Union Hospital, Jilin University, Changchun 130033, P. R. China;cNo. 1 Affiliated Hospital, Jilin University, Changchun 130022, P. R. China
Abstract:To evaluate the effect of anti-HER-2 × anti-CD3 bi-specific antibody (BsAb) on the growth of HER-2/neu-expressing human gastric carcinoma in vitro and in vivo, an MTT assay was carried out to test the inhibitive rates of herceptin, anti-CD3 and BsAb antibodies on SGC-7901 gastric carcinoma cells. Immunocytochemistry methods were used to test the HER-2 level of SGC-7901. Nude mice models were employed to test the effect of HER-2 CD3 BsAb combined with effector cells( peripheral blood lymphatic cells of healthy human beings) on the growth of tumors in animals. Compared with that of the untreated control group, the tumor cell growth rates in vitro and in vivo will both be significantly inhibited when treated with effector cells combined with anti-CD3 McAb, herceptin or HER2 CD3 BsAb (p <0. 05), and the growth inhibition is the most remarkable in the group treated with HER2 CD3 BsAb combined with effector cells. The growth of tumor xenografts will also be significantly inhibited in the group treated with HER2CD3 BsAb combined with effector cells when compared with that in the group treated with anti-CD3 McAb or the group treated with herceptin combined with effector cells(p < 0. 05). We can conclude that HER-2/neu is possibly a useful target for immunotherapy of gastric carcinoma, and anti-HER2 × anti-CD3 BsAb has evident anti-tumor efficacy both, in vitro and in vivo.
Keywords:HER-2/neu  Human gastric carcinoma  Nude mice
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