| 反相高效液相色谱分析杨梅叶中抗氧化成分黄酮类化合物 |
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| 引用本文： | 邹耀洪.反相高效液相色谱分析杨梅叶中抗氧化成分黄酮类化合物[J].分析化学,1998,26(5):531-534. |
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| 作者姓名： | 邹耀洪 |
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| 作者单位： | 常熟高等专科学校化学系!常熟215500(邹耀洪)，增城市东成日化公司!增城511370现在天津经济技术开发区(李桂荣) |
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| 摘 要： | 以反相高效液相色谱法，用ＲＰ－Ｃ１８柱为固定相，甲醇－水－甲酸为流动相，辅之以二极 管阵列紫外检测技术，从杨梅叶中分离和制备了四种黄酮酸忒和一种黄酮醇，经波谱分析确 定了化学结构，并以蒽为内标测定了杨梅叶中五种黄酮类化合物的含量．用油脂氧化酸败测 定仪（Ｍｅｔｒｏｈｍ Ｒａｎｃｉｍａｔ）测定抗氧化性能，５种黄酮类化合物均为优良的天然抗氧化剂。
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| 关 键 词： | 杨梅叶 黄酮类化合物 天然 抗氧化剂 HPLC 分析 |
Analysis of Flavonoid as Antioxidant in Myrica Rubra Leaf With Reversed-phase High Performance Liquid Chromatography |
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| Zou Yaohong.Analysis of Flavonoid as Antioxidant in Myrica Rubra Leaf With Reversed-phase High Performance Liquid Chromatography[J].Chinese Journal of Analytical Chemistry,1998,26(5):531-534. |
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| Authors: | Zou Yaohong |
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| Abstract: | Four flavonol glycosides and one flavonol were separated and collected from myrica rubra leaf using reversed-phase high performance liquid chromatography with RP-C18 column as stationary phase and methanol-water-formic acid as mobile phase. The chemical structure of five flavonoids in myrica rubra leaf was identified by spectral analysis and their content was determined by reference to internal standard anthracene. Their antioxidant activity was determined in a rancidity analyzer and results showed that these five flavonoids are active natural antioxidants. |
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| Keywords: | Reversed-phase high performance liquid chromatography myrica rubra leaf flavonoid natural antioxidant |
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