| 高速逆流色谱分离与鉴定鹿药中黄酮类化合物 |
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| 引用本文： | 赵淑杰,韩梅,韩忠明,李彦颖,杨利民.高速逆流色谱分离与鉴定鹿药中黄酮类化合物[J].分析化学,2009,37(9). |
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| 作者姓名： | 赵淑杰 韩梅 韩忠明 李彦颖 杨利民 |
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| 作者单位： | 吉林农业大学中药材学院,长春,130118 |
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| 基金项目： | 吉林省科技发展计划重大项目 |
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| 摘 要： | 采用高速逆流色谱(HSCCC)与其它色谱联用的方法分离纯化鹿药中的化学成分,得到5个黄酮类化合物: 5,7,3′,4′-四羟基-3-甲氧基-8-甲基黄酮(1)、 8-甲基木犀草素(2)、 3′-甲氧基木犀草素(3)、 木犀草素(4)和槲皮素(5),它们均为首次自该种及该属植物中分离得到.HSCCC以V(氯仿)∶ V(甲醇)∶ V(水)=4∶ 3∶ 2混合液为溶剂,上相为固定相,下相为流动相, 分离纯化得到3个分别含化合物1, 4和5的主要部分,经HPLC检验纯度分别为98.3%, 96.7%和95.3%;还有1个含有化合物2和3的较纯部分,通过Sephadex LH-20柱色谱,以V(甲醇)∶ V(水)=1∶ 1混合液洗脱将二者分离.通过理化性质及紫外、红外、质谱、核磁等波谱分析确定化合物结构.
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| 关 键 词： | 鹿药 高速逆流色谱 黄酮类化合物 木犀草素 槲皮素 |
Separation and Identification of Flavonoids from Smilacina Japonica by High Speed Counter-Current Chromatography |
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| ZHAO Shu-Jie,HAN Mei,HAN Zhong-Ming,LI Yan-Ying,YANG Li-Min.Separation and Identification of Flavonoids from Smilacina Japonica by High Speed Counter-Current Chromatography[J].Chinese Journal of Analytical Chemistry,2009,37(9). |
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| Authors: | ZHAO Shu-Jie HAN Mei HAN Zhong-Ming LI Yan-Ying YANG Li-Min |
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| Institution: | ZHAO Shu-Jie,HAN Mei,HAN Zhong-Ming,LI Yan-Ying,YANG Li-Min(College of Chinese Medicinal Materials,Jilin Agricultural University,Changchun 130118) |
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| Abstract: | Five flavoniods including 5,7,3',4'-tetrahydroxy-3-methoxy-8-methylflavone(1),8-methylluteolin(2),3'-methoxyluteolin(3),luteolin(4) and quercetin(5) were isolated and purified by high speed counter-current chromatography(HSCCC) and other chromatography from the rhizomes of Smilacina japonica.All these compounds were isolated for the first time from the species of the Smilacina genus.Preparative HSCCC with a two phase solvent system composed of chloroform-methanol-water(4:3:2,V/V),the upper phase as the stat... |
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| Keywords: | Smilacina japonica high-speed counter-current chromatography flavonoids luteolin quercetin |
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