| 基于银纳米粒子构建荧光传感平台用于核酸检测 |
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| 引用本文： | 张瑛洧,李海龙,孙旭平.基于银纳米粒子构建荧光传感平台用于核酸检测[J].分析化学,2011,39(7):998-1002. |
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| 作者姓名： | 张瑛洧 李海龙 孙旭平 |
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| 作者单位： | 中国科学院长春应用化学研究所电分析国家重点实验室;中国科学院研究生院; |
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| 基金项目： | 中国国家重点基础研究发展计划(No.2011CB935800)资助 |
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| 摘 要： | 报道了基于银纳米粒子构建的荧光传感平台,并用于核酸检测.此荧光传感平台对核酸检测基于以下策略:首先,荧光团标记的单链DNA探针被吸附到银纳米粒子的表面,荧光团与银纳米粒子近距离接触,发生荧光猝灭;加入与探针DNA序列互补的目标DNA,两者杂交形成双链DNA,并从银纳米粒子的表面脱离,荧光得到恢复.这种银纳米粒子构建的荧...
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| 关 键 词： | 核酸检测 荧光传感平台 银纳米粒子 |
| 收稿时间： | 25 February 2011 |
Silver Nanoparticles as a Fluorescent Sensing Platform for Nucleic Acid Detection |
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| ZHANG Ying-Wei,LI Hai-Long,SUN Xu-Ping.Silver Nanoparticles as a Fluorescent Sensing Platform for Nucleic Acid Detection[J].Chinese Journal of Analytical Chemistry,2011,39(7):998-1002. |
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| Authors: | ZHANG Ying-Wei LI Hai-Long SUN Xu-Ping |
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| Institution: | aState State Key Lab of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China;bGraduate School of the Chinese Academy of Sciences, Beijing 100039, China |
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| Abstract: | In this study, for the first time, we report on the use of Ag nanoparticles (AgNPs) as an effective fluorescent sensing platform for nucleic acid detection. The general concept used in this approach is based on adsorption of the FAM (fluorescein)-labeled single-stranded DNA (ssDNA) probe by AgNP, which is accompanied by substantial fluorescence quenching of FAM due to their close proximity, followed by specific hybridization with its target to form a double-stranded DNA (dsDNA) and revcover the fluorescene of FAM. The use of this sensing platform to differentiate complementary and mismatched sequences is also demonstrated. |
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| Keywords: | Nucleic acid detection Fluorescent sensing platform Ag nanoparticle |
| 本文献已被 CNKI 万方数据 ScienceDirect 等数据库收录！ |
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