| 不锈钢宽口径填充毛细管液相色谱柱的制备及评价 |
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| 引用本文： | 唐意红,朱道乾,关亚风.不锈钢宽口径填充毛细管液相色谱柱的制备及评价[J].分析化学,2001,29(10):1228-1232. |
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| 作者姓名： | 唐意红 朱道乾 关亚风 |
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| 作者单位： | 中国科学院大连化学物理研究所 |
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| 基金项目： | 国家自然科学基金资助项目（No．29875031） |
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| 摘 要： | 设计了一种零死体积的柱尾连接方式和一种能使匀浆填料均匀进入色谱柱管内的锥形储料池。详述了以反相 ＯＤＳ为固定相，制备高效稳定的不锈钢宽口径（０．８ ｍｍＩ．Ｄ．）填充毛细管液相色谱柱的方法。系统地考察了填装方向、加压溶剂、匀浆溶剂、匀浆浓度和填装压力等不同因素对柱效的影响。在４０ＭＰａ压强和优化条件下，可重复制得柱效达理论塔板数为７．５×１０４／ｍ。对所测试的样品，色谱峰不对称因子为１．０３～１．１０。
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| 关 键 词： | 填充毛细管液相色谱柱 柱制备 柱尾结构 色谱柱填充池 |
| 修稿时间： | 2000年11月26 |
Preparation and Evaluation of Stainless-Steel Wide-Bore Packed Capillary High Performance Liquid Chromatographic Columns |
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| Tang Yihong,Zhu Daoqian,Guan Yafeng.Preparation and Evaluation of Stainless-Steel Wide-Bore Packed Capillary High Performance Liquid Chromatographic Columns[J].Chinese Journal of Analytical Chemistry,2001,29(10):1228-1232. |
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| Authors: | Tang Yihong Zhu Daoqian Guan Yafeng |
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| Abstract: | The zero-dead volume union ends and conical slurry reservoir for microbore columns were designed. A detailed procedure for the preparation of highly efficient and stable stainless-steel microbore columns with reversed- phase ODS is described. The induence of packing direction, packing solvent, slurry solvent, slurry concentration and packing pressure has been studied systematically. Columns with efficiency of 75,000/m (numbar of theoretical plate) were prepared reproducibly at 40MPa packing pressure and optimized conditions. The peak asymmetric factor was 1 .03 - 1. 10 for all the compoundss tested. |
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| Keywords: | Packed capillary liquid chromatographic column column preparation column end structure packing reservoir |
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