| 停流—荧光反应速率仪及其应用于测定维生素B1 |
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| 引用本文： | 杨屹,蔡汝秀.停流—荧光反应速率仪及其应用于测定维生素B1[J].分析化学,1993,21(3):360-363. |
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| 作者姓名： | 杨屹 蔡汝秀 |
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| 作者单位： | 武汉大学化学系，武汉大学化学系，武汉大学化学系，武汉大学化学系 武汉 430072，武汉 430072，武汉 430072，武汉 430072 |
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| 摘 要： | 本文利用国产930型荧光光度计作为检测器,采用自已制作的流通池,配以蠕动泵及记录仪,组装成停流-荧光动力学分析装置,并用以进行了停流荧光反应速率法测定维生素B_1的研究,利用Hg~(2+)离子在碱性介质中将硫胺素(维生素B_1)氧化为硫胺荧的动力学反应,测定了维生素B_1片剂中维生素B_1的含量。结果表明,这套仪器用于动力学分析,操作简便、省时,维生素B_1的测定结果与标准值基本相符,相对标准偏差为1.28％,其准确度和重现性均令人满意。
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| 关 键 词： | 荧光反应速率 维生素B1 停流技术 |
Stopped-flow Fluorescence Reaction Rate Analyzer and Its Application for the Determination of Thiamine |
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| Yang Yi. Cai Ruxiu,Huang Houping,Zeng Yun''''e.Stopped-flow Fluorescence Reaction Rate Analyzer and Its Application for the Determination of Thiamine[J].Chinese Journal of Analytical Chemistry,1993,21(3):360-363. |
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| Authors: | Yang Yi Cai Ruxiu Huang Houping Zeng Yun'e |
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| Abstract: | A stopped-flow fluorescence reaction rate analyzer was developed. It consisted of a fluorescence photometer (Model 930) with a self-made flow-through cell, a peristaltic pump and a recorder. The reaction involving the oxidation of thiamine by Hg2- in basic solutions to fluorescent thiochrome was studied. The content of thiamine in vitamin B1 tablet was determined with satisfactory results(RSD
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| Keywords: | Stopped-flow fluorescence reaction rate analyzer Stopped-flow fluorescence reaction rate method Thiamine |
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