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| 蛋白质组学技术筛选与鉴定镉诱导下褐云玛瑙螺肝脏的差异蛋白质 | |
| 引用本文： | 陈海滨,黄琳,黄清育,凌雪萍,朱锋,黄河清.蛋白质组学技术筛选与鉴定镉诱导下褐云玛瑙螺肝脏的差异蛋白质[J].分析化学,2009,37(6). |
| 作者姓名： | 陈海滨 黄琳 黄清育 凌雪萍 朱锋 黄河清 |
| 作者单位： | 1. 厦门大学生命科学学院生物化学与生物技术学系 2. 厦门大学生命科学学院生物化学与生物技术学系;化学化工学院化学生物学系,厦门,361005 3. 厦门大学生命科学学院生物化学与生物技术学系;海洋与环境科学学院近海海洋科学国家重点实验室;化学化工学院化学生物学系,厦门,361005 |
| 基金项目： | 国家自然科学基金，福建省高校创新研究团队基金 |
| 摘 要： | 比较丙酮/TCA沉淀法和直接裂解法,优化提取与分离褐云玛瑙螺(Achatina fulica, AF)肝脏全蛋白.采用丙酮/TCA沉淀法,可获得约600个蛋白质斑点.用0.5 mg/L CdCl2溶液浸泡后的去梗小白菜喂养AF,并作为镉盐诱导AF肝脏表达应激蛋白质的实验材料.采用蛋白质组学技术筛选出由镉盐诱导AF肝脏表达的14个差异蛋白质斑点,并用肽质量指纹图谱技术(peptide mass fingerprinting, PMF)和数据库比对法初步鉴定出7种差异蛋白质,其中部分为热激蛋白(heat shock protein)、甲基转移酶(Methyltransferase)、三磷酸腺苷结合盒子转运体(ABC transporter)、钼酸盐转运子亚基(molybdate transporter subunit)和磷酸甘油酸变位酶(phosphoglycerate mutase).差异蛋白质均参与镉盐代谢,并适合作为监测土壤或食物中镉污染程度及危害性的指示蛋白质. |
| 关 键 词： | 镉盐 差异蛋白质 蛋白质组学 污染监测 肝脏 |
Selection and Identification of Differential Proteins of Liver with Proteomics Techniques in Achatina Fulica Under Induction of Cadmium |
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| CHEN Hai-Bin,HUANG Lin,HUANG Qing-Yu,LING Xue-Ping,ZHU Feng,HUANG He-Qing.Selection and Identification of Differential Proteins of Liver with Proteomics Techniques in Achatina Fulica Under Induction of Cadmium[J].Chinese Journal of Analytical Chemistry,2009,37(6). | |
| Authors: | CHEN Hai-Bin HUANG Lin HUANG Qing-Yu LING Xue-Ping ZHU Feng HUANG He-Qing |
| Institution: | 1Department of Biochemistry and Biotechnology;School of Life Sciences;2State Key Laboratory of Marine EnvironmentalScience;College of Oceanography and Environmental Science;3The Key Lab.of Chemical Biology of Fujian Province;College of Chemistry & Chemical Engineering;Xiamen University;Xiamen 361005 |
| Abstract: | In comparison with direct lysis,the precipitation analysis of acetone/trichloroacetic acid was selected to optimize and separate whole proteins from the liver tissue of Achatina fulica(AF).By this method,approximately 600 protein spots in gel were obtained.Using a cabbage exposed by 0.05 mg/L CdCl2 as AF food,the liver was selected to reveal the stress proteins in the AF exposed to acute cadmium toxicity.Fourteen of differential protein spots in AF liver were obtained by proteomics,and then seven of these s... |
| Keywords: | Cadmium differential proteins proteomics contamination monitoring liver |
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