| 小牛胸腺脱氧核糖核酸诱导中性红聚集的机理及其影响因素 |
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| 引用本文： | 黄新华,李原芳,奉萍,黄承志.小牛胸腺脱氧核糖核酸诱导中性红聚集的机理及其影响因素[J].分析化学,2000,28(6):682-686. |
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| 作者姓名： | 黄新华 李原芳 奉萍 黄承志 |
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| 作者单位： | 西南师范大学环境化学研究所,重庆,400715 |
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| 基金项目： | 国家自然科学基金!29875019 |
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| 摘 要： | 在ｐＨ７．６￣７．８和低离子强度条件下，中性红（ＮＲ）与小牛脱氧核糖核酸（ｃｔＤＮＡ）作用产生以５３５ｎｍ为特征的共振光散射增强（ＥＲＬＳ）光谱。机理研究表明，这种ＥＲＬＳ是由于ＤＮＡ诱导中性红的聚集所致。确定了最佳聚集条件，考察了聚集的影响因素。
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| 关 键 词： | 共振光散射 中性红 小牛胸腺 DNA 聚集机理 |
The Aggregation of Neutral Red Encouraged by Deoxyribonucleic Acids And Its Depending Factors |
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| Huang Xinhua,Li Yuanfang,Feng Ping,Huang Chengzhi.The Aggregation of Neutral Red Encouraged by Deoxyribonucleic Acids And Its Depending Factors[J].Chinese Journal of Analytical Chemistry,2000,28(6):682-686. |
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| Authors: | Huang Xinhua Li Yuanfang Feng Ping Huang Chengzhi |
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| Abstract: | On the conditions of pH 7.6~7.8 and low ionic strength,the interaction of neutral red (NR) with deoxyribonucleic acids (DNA) results in enhanced resonance light-scattering (ERLS),CHARACterized by 535 nm RLS peak.Mechanism study shows that the ERLS is due to the induced aggregation of NR by DNA.Involving factors on the aggregations and optimal conditions were discussed. |
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| Keywords: | Enhancement of resonance light scattering neutral Red deoxynucleotide acids |
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