| 光纤荧光免疫分析系统的研制及应用 |
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| 引用本文： | 何俊,但德忠,李坤,张本,袁粒军,蒲思慧,李毓琦.光纤荧光免疫分析系统的研制及应用[J].分析化学,2005,33(10):1409-1412. |
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| 作者姓名： | 何俊 但德忠 李坤 张本 袁粒军 蒲思慧 李毓琦 |
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| 作者单位： | [1]四川大学化学学院,成都610041 [2]四川大学建筑与环境学院,成都610059 [3]四川大学附二院儿科病毒实验室,成都610041 [4]四川大学药学院,成都610041 |
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| 摘 要： | 将光纤传感技术、荧光分析与免疫分析技术结合，自行设计了Y型分叉光纤、光强可调的光纤固定架及性能优良的荧光测定池，建立了新型光纤荧光免疫系统。系统性能可靠，操作简便，既可进行普通的荧光分析，又可进行光纤荧光免疫分析。将系统用于临床血清标本中肺炎支原体抗体的测试，结果与荧光显微镜法和ELISA法结果一致。该新型光纤荧光免疫系统在临床、环保等领域有广泛的应用前景。
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| 关 键 词： | 免疫分析 光纤传感器 荧光分析 肺炎支原体抗体 |
| 收稿时间： | 10 9 2004 12:00AM |
| 修稿时间： | 2004-10-092005-04-18 |
Novel Optic Fiber Fluoroimmunoassay System and Its Application |
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| He Jun, Dan Dezhong, Li Kun, Zhang Ben, Yuan Lijun, Pu Sihui, Li Yuqi.Novel Optic Fiber Fluoroimmunoassay System and Its Application[J].Chinese Journal of Analytical Chemistry,2005,33(10):1409-1412. |
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| Authors: | He Jun Dan Dezhong Li Kun Zhang Ben Yuan Lijun Pu Sihui Li Yuqi |
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| Institution: | 1.Chemical Institute, Sichuan University, Chengclu 610041;2. Institute of Architecture and Environment, Sichuan University, Chengdu 610065;3. Department of Paediatrics, West China Second Hospital , Sichuan University, Chengdu 610041; 4; Pharmic Institute, Sichuan University, Chengdu 610041 |
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| Abstract: | A novel analytical system has been developed with the incorporation of three advanced techniques,namely,fiber-optic sensor,fluorescent analysis technique and immunoassay.A Y-type crotch optic fiber and its fixed support as well as fluorescent measure cassette for the analytical system were designed well with good performance.Immunoglobulin(IgG) antibody labeled by fluorescein isothiocyanate(FITC) as a fluorescent tag has been employed for the determination of human serum specimen by the analytical system.Adopting the novel analytical system to determine the dinical speimen of 35 samples,the anurary is consistent with two other methods.The results show that the fiber-optic fluorescence immunoassay system is reasonable;the function is dependable;the operation is simple and fleet.Also the instrument is economical and easy universalized etc.It will have extensive application in the fields of clinic diagnosis,environmental protection and food safety. |
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| Keywords: | Immunoassay fiber-optic sensor fluorescence analysis mycoplasma pneumoniae antibody |
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