| 压电免疫传感器用于C2型葡萄球菌肠毒素的测定 |
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| 引用本文： | 高志贤,张超.压电免疫传感器用于C2型葡萄球菌肠毒素的测定[J].分析化学,1997,25(9):1061-1063. |
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| 作者姓名： | 高志贤 张超 |
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| 作者单位： | 军事医学科学院卫生学环境医学研究所!天津300050(高志贤，陶桂全，李新如)，清华大学精密仪器与机械学系!北京100084(张超)，中国科学院微生物研究所!北京100080(黎高翔) |
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| 基金项目： | 国家自然科学基金，天津21世纪青年科学基金资助课题。 |
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| 摘 要： | 报道了用蛋白Ａ－金电极法将抗体固化在３．５８ＭＨｚ和１０ＨｚＡＴ切割的石英晶体上，制作的可重复使用的压电免疫传感器，测定Ｃ２型葡萄球菌肠毒素。最适ＰＨ值为７，并用不同的方法测试了晶体片上的抗体固化情况。
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| 关 键 词： | 压电晶体 免疫传感器 碳2型 葡萄球菌 肠毒素 SE |
Detection of Staphylococcus Enterotoxin With a Piezoelectric Immunosensor |
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| Gao Zhixian,Tao Guiquan,Li Xinru.Detection of Staphylococcus Enterotoxin With a Piezoelectric Immunosensor[J].Chinese Journal of Analytical Chemistry,1997,25(9):1061-1063. |
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| Authors: | Gao Zhixian Tao Guiquan Li Xinru |
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| Abstract: | A reusable piezoelectric immunosensor has been developed for detection of Staphy- lococcusEnterotoxin by immobilization at antibody with protein A method to the gold elec- trode of 3. 58 MHz AT-cut quartz crystal. The optimum pH vaule was 7. Immobilized anti- body on the surface of crystal electrode was tested with different methods, and observed that after antigen assay the sensor could be used six times by 8 mol/L urea elution. |
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| Keywords: | Piezoelectric crystal immunosensor protein A |
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