| 电喷雾串联质谱分析附子炮制中的化学成分变化 |
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| 引用本文： | 越皓,皮子凤,赵宇峰,宋凤瑞,刘志强,刘淑莹.电喷雾串联质谱分析附子炮制中的化学成分变化[J].分析化学,2007,35(7):959-963. |
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| 作者姓名： | 越皓 皮子凤 赵宇峰 宋凤瑞 刘志强 刘淑莹 |
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| 作者单位： | 1. 中国科学院长春应用化学研究所,长春质谱中心,长春,130022;中国科学院研究生院,北京100039
2. 中国科学院长春应用化学研究所,长春质谱中心,长春,130022 |
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| 基金项目： | 国家自然科学基金(No30472134)，中国科学院知识创新工程重要方向项目(NoKGCX2-SW-213-06) |
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| 摘 要： | 利用电喷雾质谱方法(ESI-MS)分析了附子加辅料(甘草)炮制前后水煎液中二萜类生物碱在种类和含量方面的变化,通过加入内标化合物,建立了电喷雾质谱的半定量分析方法。此方法具有快速、准确、灵敏的特点,能够更加全面地反映中药配伍炮制过程中多种化学成分的含量变化,并能根据电喷雾串联质谱的分析结果鉴定配伍后产生的新的化学成分,在共煎液中的次乌头碱、中乌头碱和乌头碱的相对含量分别是单煎液中的5.67%、4.05%和4.88%。通过研究附子与甘草的单煎液、共煎液以及药渣中化学成分的变化,揭示了甘草作为辅料,在炮制过程中对附子减毒作用机理。
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| 关 键 词： | 附子 炮制机理 电喷雾串联质谱 二萜类生物碱 |
| 修稿时间： | 2006-10-282007-03-30 |
Analysis of Norditerpenoid Alkalods in Processing Radix Aconiti Lateralis Preparata with Radix Glycyrrhizae Preparata by Electrospray Ionization Tandem Mass Spectrometry |
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| Yue Hao,Pi Zi-Feng,Zhao Yu-Feng,Song Feng-Rui,Liu Zhi-Qiang,Liu Shu-Ying.Analysis of Norditerpenoid Alkalods in Processing Radix Aconiti Lateralis Preparata with Radix Glycyrrhizae Preparata by Electrospray Ionization Tandem Mass Spectrometry[J].Chinese Journal of Analytical Chemistry,2007,35(7):959-963. |
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| Authors: | Yue Hao Pi Zi-Feng Zhao Yu-Feng Song Feng-Rui Liu Zhi-Qiang Liu Shu-Ying |
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| Institution: | 1. Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun Center of Mass Spectrometry, Changchun 130022; 2. Graduate School of the Chinese Academy of Sciences, Beijing 100039 |
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| Abstract: | Norditerpenoid alkaloids in the processing of Radix aconiti lateralis preparata with Radix glycyrrhizae preparata was studied by electrospray ionization tandem mass spectrometry. A method of ESI-MS couple with internal standard was applied for semiquantitative analysis of norditerpenoid alkaloids before and after processing. The combined-decoction-to single-decoction-ratios of the relative abundance of toxic hypaconitine, mesaconitine and aconitine were 5.67%, 4.05% and 4.88%, respectively. The chemical changes of processing can been comprehensive observed by ESI-MS/MS analysis. The scientific basis of reducing the toxicity of Radix aconiti lateralis preparata with Radix glycyrrhizae preparata was enlightened by compareing with the single-decotion, the combined-decoction and residue for the compatibility of Radix aconiti lateralis preparata and Radix glycyrrhizae preparata. |
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| Keywords: | Radix aconiti lateralis preparat process electrospray ionization-mass spectrometry norditerpenoid alkaloids |
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