| 固相萃取-色谱测定水、沉积物及土壤中氯霉素和3种四环素类抗生素 |
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| 引用本文： | 刘虹,张国平,刘丛强.固相萃取-色谱测定水、沉积物及土壤中氯霉素和3种四环素类抗生素[J].分析化学,2007,35(3):315-319. |
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| 作者姓名： | 刘虹 张国平 刘丛强 |
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| 作者单位： | 1. 中国科学院地球化学研究所环境地球化学国家重点实验室,贵阳,550002;中国科学院研究生院,北京,100039
2. 中国科学院地球化学研究所环境地球化学国家重点实验室,贵阳,550002 |
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| 基金项目： | 中国科学院创新团队国际合作伙伴计划，中国科学院重要方向项目(No.KZCX3-SW-140)资助 |
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| 摘 要： | 建立了固相萃取-高效液相色谱(UV)检测水、沉积物和土壤中的氯霉素、土霉素、四环素和金霉素4种抗生素的方法。水样、土壤和沉积物样品的前处理采用EDTA-McIlvaine缓冲溶液提取,用SAX-HLB串联小柱纯化和富集,用丙酮(含10%甲醇)洗脱进一步减小天然有机质的影响。采用乙腈和0.01mol/L草酸溶液作为流动相,8min内分离4种抗生素。测定了贵阳市阿哈湖、南明河和乌江渡水库的水样及沉积物,均有抗生素检出。
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| 关 键 词： | 固相萃取 高效液相色谱 氯霉素 四环素类 抗生素 |
| 修稿时间： | 2006-07-032006-10-07 |
Determination of Chloramphenicol and Three Tetracyclines by Solid Phase Extraction and High Performance Liquid Chromatography-Ultraviolet Detection |
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| Liu Hong,Zhang Guo-Ping,Liu Cong-Qiang.Determination of Chloramphenicol and Three Tetracyclines by Solid Phase Extraction and High Performance Liquid Chromatography-Ultraviolet Detection[J].Chinese Journal of Analytical Chemistry,2007,35(3):315-319. |
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| Authors: | Liu Hong Zhang Guo-Ping Liu Cong-Qiang |
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| Institution: | 1.State Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of Sciences, Guiyang 550002 ;2.Graduate School of Chinese Academy of Sciences, Beifing 100039 |
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| Abstract: | A method for determination of chloramphenicol, oxytetracycline, tetracycline and chlortetracycline in water, sediment and soil, using solid phase extraction with high performance liquid chromatography-UV detection was developed. The four antibiotics in water, sediment and soil were extracted using a mixture of EDTA and McIlvaine buffer (citric acid and sodium orthophosphate), then cleaned-up and pre-concentrated by a tandem method using strong anion exchanger (SAX) cartridge and hydrophilic-liphophilic balance (HLB) cartridge and then eluted by acetone (including 10% methanol) to depress the interference of nature organic compounds. Using acetonitrile and 0.01 mol/L oxalic acid as the mobile phase, the four antibiotics were separated within 8 min.This method was applied to the analysis of antibiotics in lake water, river water and lake sediment. |
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| Keywords: | Solid phase extraction high performance liquid chromatography chloramphenicol tetracyclines antibiotics |
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