| 核酸适配体识别-荧光法检测赭曲霉素A |
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| 引用本文： | 段诺,吴世嘉,王周平.核酸适配体识别-荧光法检测赭曲霉素A[J].分析化学,2011,39(3):300-304. |
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| 作者姓名： | 段诺 吴世嘉 王周平 |
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| 作者单位： | 江南大学食品学院食品科学与技术国家重点实验室; |
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| 基金项目： | 国家“863”计划(No.2008AA10Z419); 国家自然科学基金(No.20805019); 教育部博士点基金新教师计划(No.20070295014); 江苏省自然科学基金(No.BK20081603); “111”引智计划((No.B07029)资助项目 |
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| 摘 要： | 建立了核酸适配体识别-荧光探针技术检测赭曲霉素A(OTA)的新方法.基于微孔板上固定的核酸适配子与目标物质OTA结合时构象发生变化,导致预先与其互补杂交的FAM标记短链DNA解离,引起荧光信号发生变化,据此可实现对OTA的定量检测.当微孔板包被亲和素浓度为25 mg/L、适配子浓度为50 nmol/L,FAM标记互补短...
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| 关 键 词： | 核酸适配体 荧光 分子识别 赭曲霉素A |
| 收稿时间： | 18 July 2010 |
An Aptamer-based Fluorescence Assay for Ochratoxin A |
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| DUAN Nuo,WU Shi-Jia,WANG Zhou-Ping.An Aptamer-based Fluorescence Assay for Ochratoxin A[J].Chinese Journal of Analytical Chemistry,2011,39(3):300-304. |
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| Authors: | DUAN Nuo WU Shi-Jia WANG Zhou-Ping |
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| Institution: | a State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China |
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| Abstract: | A novel analytical method for the detection of Ochratoxin A(OTA) was established based on the aptamer recognition and fluorescent probe technology.The method was developed according to the fact that when the immobilized aptamer bond to the target OTA,it can induce the conformation change of aptamer and result in the dissociation of the carboxy-fluorescein(FAM)-tagged complementary DNA chain from aptamer,and finally lead to the fluorescent signals change.Based on it,OTA can be quantified.All the condition fa... |
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| Keywords: | Aptamer Fluorescence analysis Ochratoxin A |
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