氢化物发生-分光光度法测定中药中微量锗

研究了在L 半胱氨酸存在下 ,锗与硼氢化钠反应产生锗化氢挥发分离 ,再用显色剂 2 (5 NO2 2 吡啶偶氮 ) 5 二乙氨基酚 (5 NO2 PADAP) ,次氯酸钠 ,六次甲基四胺的混合液吸收显色 ,有色溶液在 5 6 4nm处有最大吸收 ;摩尔吸光系数为 1 84× 10 5L·mol-1·cm-1,锗在 0～ 5 0 0 μg/L范围内服从比尔定律 ;样品标准加入回收率为 89%～ 97% ;相对标准偏差小于 6 .3% ;用本法对中药材中微量锗的测定 ,结果令人满意

Spectrophotometric Determination of Trace Germanium in Chinese Medicine by Hydride Generation Technique

The hydride generation technique was used to determination of germanium in the presence of L-cysteine.The GeH4 was absorbed and coloured by a mixed solution of 2-(5-NO2-2-pyridylazo)-5-diethylaminophenyl(5-NO2-PADAP),NaClO and (CH2)6NH+.The maximum absorption of the colour complex was at 564nm.Its apparent molar absorption coefficient was 1.84×105L·mol-1·cm-1.Beer＇s law was obeyed for 0～500μg/L germanium.The recoveries of spiked samples were in the range of 89%～97%,while the relative standard deviation was less than 6.3%.This method is sensitive,simple and reproducible.The method has been applied to the determination of Ge in the chinese medicine with satisfactory result.