阴离子型胶束液相色谱的溶质保留行为

以SDS阴离子表面活性剂作流动相，酸性、中性及两性药物为受试药物，运用三相平衡理论考察影响阴离子型胶束液相色谱（AMLC）溶质保留行为的几个因素。保留由溶质与胶束相及修饰后固定相的综合作用决定。有机调节剂正丙醇的加入改变了溶质从水相到固定相或到胶束相的平衡，保留取决于溶质疏水性和静电性间的平衡。此外对羟基苯甲酸酯类同系物的亲脂性与3种细菌最小抑菌浓度具有显著相关性，提示其抑菌机理主要取决于药物与生物膜的亲和性。

The Retention Behavior of Solutes in Anionic Micellar Liquid Chromatography

The retention characteristics of solutes in anionic micellar liquid chromatography (AMLC) were investigated in terms of three-phase equilibrium theory, using an anionic surfactant(sodium dodecyl sulfate)solution as mobile phase. Acidic, basic and amphoteric solutes were investigated. The retention of solutes was depended on the assembled interaction of solutes with the micelles in the mobile phase and with the modified stationary phase. When adding organic modifier, the partitioning equilibria between aqueous phase and modified stationary phase, aqueous phase and micellar phase were significantly altered. Also, the accordingly retention behavior of solutes was managed by the balance of hydrophobic and electrostatic interaction. Moreover, there was good correlation between lipophilicity from MLC, immobilized artificial membrane (IAM) and n-octanol/buffer, and minimal inhibitory concentration of three microbes for p-hydroxybenzoic ester anologs and it suggestive that analogs their inhibitory mechanism may be associated with membrane affinity.