烟叶和烟草料液中氨基酸的直接检测及碳水化合物的去除

建立了高效阴离子交换色谱-积分脉冲安培法(HPAEC-IPAD)直接检测烟叶和烟草料液中氨基酸的方法。利用离线除糖的方法,去除烟叶和烟草料液中大量干扰糖类,包括葡萄糖、果糖和蔗糖及麦芽低聚糖等。采用AminoPac PA10阴离子交换柱,以NaOH和NaAc的强碱性溶液为淋洗液,采用梯度洗脱,流速为0.25 mL/min;积分脉冲安培法对氨基酸进行检测,回收率可达76%~105%。此方法可以有效的解决烟叶、烟草料液等含糖量高的样品中糖类化合物的干扰,对氨基酸实现灵敏、准确的定量分析。

Direct Determination of Amino Acids and Off-line Elimination of Carbohydrates in Tobacco and Tobacco Feed Liquid Samples

A method was established for the direct determination of amino acids in tobacco and tobacco feed liquid by high performance anion exchange chromatography with integrated pulsed amperometric detection(HPAEC-IPAD).Using an off-line method,high concentrations of carbohydrates which can significantly interfere with amino acids determination were eliminated, including glucose,fructose,sucrose,oligosaccharides,and so on.The separation was carried out on an AminoPac PA10 anion-exchange column with NaOH/NaAc gradient eluent at a flow rate of 0.25 mL/min.The determination was perfomed with integrated pulsed amperometric detector.The recoveries were in the range of 76%-105%.The method can be an efficient tool for the interfere elimination of high concentration of carbohydrates in samples such as tobacco and tobacco feed liquid,and accurate determination of amino acids.