| 清开灵注射液HPLC/ELSD指纹图谱建立及质量相关性研究 |
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| 引用本文： | 曹进,徐燕,张永知,王义明,罗国安.清开灵注射液HPLC/ELSD指纹图谱建立及质量相关性研究[J].分析化学,2004,32(4):469-473. |
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| 作者姓名： | 曹进 徐燕 张永知 王义明 罗国安 |
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| 作者单位： | 清华大学药物研究所,北京,100084;清华大学药物研究所,北京,100084;清华大学药物研究所,北京,100084;清华大学药物研究所,北京,100084;清华大学药物研究所,北京,100084 |
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| 基金项目： | 国家 973重点基础研究项目 (No .G19990 5 44 0 4)，科技部重大专项 (No .2 0 0 2BA90 6A2 9 3 )资助项目 |
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| 摘 要： | 运用高效液相色谱／蒸发光散射检测器(HPLC／ELSD)建立了复方清开灵注射液的指纹图谱。对其中7种有效成分进行了定量测定，方法学考察结果良好；通过指纹图谱相似度评价软件，对10批清开灵注射液的HPLC／ELSD指纹图谱进行了相似度计算，结果表明：10批样品相似度很好。该方法为中药质量控制提供了一种可以同时实现整体定性、指标成分定量且简便易行的方法模式。
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| 关 键 词： | 高效液相色谱 蒸发光散射 清开灵 指纹图谱 |
Fingerprint Chromatogram by High-performance Liquid Chromatography with Evaporative Light Scattering Detection of QingKaiLing Injection and Its Relationship with Quality Control |
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| Cao Jin,Xu Yan,Zhang Yongzhi,Wang Yiming,Luo Guoan.Fingerprint Chromatogram by High-performance Liquid Chromatography with Evaporative Light Scattering Detection of QingKaiLing Injection and Its Relationship with Quality Control[J].Chinese Journal of Analytical Chemistry,2004,32(4):469-473. |
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| Authors: | Cao Jin Xu Yan Zhang Yongzhi Wang Yiming Luo Guoan |
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| Institution: | Cao Jin,Xu Yan,Zhang Yongzhi,Wang Yiming,Luo Guoan * |
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| Abstract: | By high performance liquid chromatography with evaporative light scattering detection(HPLC/ELSD),we established the fingerprint chromatogram of QingKaiLing injection. The method was applied to quantify 7 major bioactive components in it simultaneously with satisfactory results. Using special software, we got the HPLC/ELSD fingerprint chromatogram similarity of 10 injection and found the perfect correlation among them. The method connecting HPLC with ELSD could be used as a credible and convenient quality control method for the Chinese Medicinal Medicine. |
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| Keywords: | High performance liquid chromatography evaporative light scattering detection QingKaiLing chromatogram fingerprint |
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