| 高效液相色谱法紫外与激光诱导荧光检测蛋白质的分析对比 |
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| 引用本文： | 马丽敏,宋伦,钱俊红,张凌怡,蓝闽波,张维冰.高效液相色谱法紫外与激光诱导荧光检测蛋白质的分析对比[J].分析化学,2011,39(7):1027-1032. |
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| 作者姓名： | 马丽敏 宋伦 钱俊红 张凌怡 蓝闽波 张维冰 |
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| 作者单位： | 华东理工大学上海市功能性材料化学重点实验室分析测试中心;华东理工大学生物反应器工程国家重点实验室; |
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| 基金项目： | 国家重点基础研究发展规划(973)项目(No.2011CB910400); 上海市科委专项基金(No.10dz2220500); 华东理工大学优秀青年教师基金(No.YJ0157/45)资助项目 |
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| 摘 要： | 将鼠肝肿瘤组织蛋白及其酶解产物经4-氟-7-硝基-2,1,3-苯并氧杂恶二唑(NBD-F)在50 mmol/L硼砂缓冲溶液(pH 8.5)中室温避光衍生1h后,采用高效液相色谱-激光诱导荧光检测法(HPLC-LIF)分析(λ(ex)=473nm,λ(em)=525 mn),并与高效液相色谱-紫外检测法(HPLC-UV)...
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| 关 键 词： | 蛋白 多肽 激光诱导荧光 高效液相色谱 |
Comparative Study on Determination of Proteins by High Performance Liquid Chromatography with Ultraviolet or Laser-Induced Fluorescence as Detector |
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| MA Li-Min,SONG Lun,QIAN Jun-Hong,ZHANG Ling-Yi,LAN Min-Bo,ZHANG Wei-Bing.Comparative Study on Determination of Proteins by High Performance Liquid Chromatography with Ultraviolet or Laser-Induced Fluorescence as Detector[J].Chinese Journal of Analytical Chemistry,2011,39(7):1027-1032. |
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| Authors: | MA Li-Min SONG Lun QIAN Jun-Hong ZHANG Ling-Yi LAN Min-Bo ZHANG Wei-Bing |
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| Institution: | MA Li-Min1,SONG Lun1,QIAN Jun-Hong1,ZHANG Ling-Yi1,LAN Min-Bo1,2,ZHANG Wei-Bing1 1(Shanghai Key Laboratory of Functional Materials Chemistry,and Research Centre of Analysis and Test,East China University of Science and Technology,Shanghai 200237) 2(State Key Laboratory of Bioreactor Engineering,Shanghai 200237) |
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| Abstract: | |
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| Keywords: | Proteins Peptides Laser-induced fluorescence High performance liquid chroma-tography |
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