| 多维色谱串联质谱分析肝癌患者血浆中的低丰度差异蛋白 |
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| 引用本文： | 肖忠华,彭咏波,邱宗荫.多维色谱串联质谱分析肝癌患者血浆中的低丰度差异蛋白[J].分析化学,2009,37(4). |
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| 作者姓名： | 肖忠华 彭咏波 邱宗荫 |
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| 作者单位： | 1. 重庆医科大学药学院,重庆,400016
2. 中国药科大学现代中药教育部重点实验室,南京,210009 |
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| 基金项目： | 国家自然科学基金，重庆市自然科学基金 |
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| 摘 要： | 建立了分析肝癌患者血浆中低丰度差异蛋白的新的多维色谱串联质谱方法.以5例肝细胞癌(HCC)患者血浆为研究对象,选用免疫亲和色谱Agilent MARS Spin Cartridge去除血浆中6种主要高丰度蛋白;离线RP-HPLC预分级血浆低丰度蛋白并收集差异表达蛋白组;液相色谱-芯片(HPLC-CHIP,包含一支纳升级Zorbax 300SB-C18富集柱和一支纳升级Zorbax 300SB-C18分析柱)富集、分离差异表达蛋白组胰酶酶解肽混合物,线性离子阱质谱在线分析,以Spectrum Mill MS Proteomics Workbench自动分析MS和MS/MS数据后在UniProtKB/SWISS-PORT数据库中进行搜索,鉴定得到27种差异蛋白,其中23种蛋白与各种疾病或肿瘤相关,IMP3、ARNT2和GRIP1可能成为诊断HCC的潜在生物标志物.
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| 关 键 词： | 肝癌 血浆蛋白 免疫亲和色谱 反相色谱 二维纳流芯片液相色谱离子阱质谱 生物标志物 |
Analysis of Low Abundance Diferential Proteins in Plasma from Hepatocellular Carcinomas Patients by Multi-dimensional Chromatography Tandem Mass Spectrometry |
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| XIAO Zhong-Hua,PENG Yong-Bo,QIU Zong-Yin.Analysis of Low Abundance Diferential Proteins in Plasma from Hepatocellular Carcinomas Patients by Multi-dimensional Chromatography Tandem Mass Spectrometry[J].Chinese Journal of Analytical Chemistry,2009,37(4). |
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| Authors: | XIAO Zhong-Hua PENG Yong-Bo QIU Zong-Yin |
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| Institution: | Chongqing Medical University of Pharmacy;Chongqing 400016;Key Laboratory of Modern Chinese Medicines;Ministry of Education;China Pharmaceutical University;Nanjing 210009 |
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| Abstract: | A multi-dimensional separation techniques was developed to analyze differential plasma proteins of immunodepleted plasma between five cases of patients with hepatocellular carcinoma(HCC) for the study and five cases of healthy volunteers for the control of plasma mixed. Samples of human plasma were depleted for six most abundance proteins,using an immunoaffinity chromatography Agilent MARS Spin Cartridge,then were separated under the preffered conditions by reversed phase LC. Over-expressed differential fra... |
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| Keywords: | Hepatocellular carcinoma plasma protein immune affinity chromatography reversed phase high performance liquid chromatography high performance liquid chromatography-chip mass sepctrometry biomarker |
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