| 重组内皮抑素的性质表征与生物活性分析 |
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| 引用本文： | 王群,季怡萍,倪嘉缵.重组内皮抑素的性质表征与生物活性分析[J].分析化学,2005,33(4):459-462. |
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| 作者姓名： | 王群 季怡萍 倪嘉缵 |
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| 作者单位： | 1. 上海交通大学药学院,上海,200030
2. 中国科学院长春应用化学研究所稀土化学与物理开放实验室,长春,130022 |
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| 摘 要： | 通过SDS—PAGE电泳、基质辅助激光解吸电离飞行时间质谱、高效液相色谱、地高辛方法、蛋白印迹方法、鸡胚尿囊膜血管生成抑制实验和透射电子显微镜法对重组内皮抑素进行纯度分析、鉴定和生物活性研究。重组内皮抑素纯度达到99％，能够抑制鸡胚尿囊膜新血管生成和人脐静脉内皮细胞ECV304增殖，引发内皮细胞的凋亡。
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| 关 键 词： | 重组内皮抑素 质谱 电镜 细胞凋亡 |
Characterization of Recombinant Endostatin and Analysis of Its Biological Activities |
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| Wang Qun,Ji Yiping,Ni Jiazuan.Characterization of Recombinant Endostatin and Analysis of Its Biological Activities[J].Chinese Journal of Analytical Chemistry,2005,33(4):459-462. |
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| Authors: | Wang Qun Ji Yiping Ni Jiazuan |
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| Abstract: | The purity and characterization of recombinant endostatin protein were studied by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, matrix-assisted laser desorption/ionization-time of flight-mass spectrometry, high performance liquid chromatography and digolan methods. The biological activities of protein were determined by chicken chorioallantoic membrane (CAM) vascular inhibition test and transmission electron microscopy assay. The protein was identified by western blot and its purity reached 99% after purification. The recombinant endostatin showed significant inhibitory effect on angiogenesis in CAM and proliferation of endothelial cell, induced apoptosis of human endothelial cell ECV304. |
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| Keywords: | Recombinant endostatin mass spectrometry electron microscope cell apoptosis |
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