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| 去甲氯胺酮半抗原及其全抗原的合成与鉴定 | |
| 引用本文： | 杨婷婷,赵春城,沈笑平,谈伯昌,邹盘坤,王怀明,刘凌云,韦超,魏万里,何金海,吴杰,张东升,赵晓联.去甲氯胺酮半抗原及其全抗原的合成与鉴定[J].分析化学,2010,38(1). |
| 作者姓名： | 杨婷婷 赵春城 沈笑平 谈伯昌 邹盘坤 王怀明 刘凌云 韦超 魏万里 何金海 吴杰 张东升 赵晓联 |
| 作者单位： | 1. 江苏省苏微微生物研究有限公司,无锡,214063 2. 无锡市公安局,无锡,214002 3. 镇江市公安局,镇江,212001 4. 江阴市公安局,江阴,214400 5. 盐城市公安局,盐城,224001 |
| 基金项目： | 江苏省社会发展项目(No.BS2007022)资助 |
| 摘 要： | 在低温条件下,去甲氯胺酮与琥珀醛酸反应,合成了半抗原羧基-去甲氯胺酮,电喷雾质谱鉴定结果表明,目标半抗原合成成功;通过碳二亚胺法将半抗原与载体蛋白偶联制备人工抗原,红外光谱法鉴定结果表明,人工抗原合成成功,基质辅助激光解析电离飞行时间质谱鉴定表明去甲氯胺酮半抗原与牛血清白蛋白的偶联比为11:1。经动物免疫,获得高效价特异性多克隆抗体,抗血清效价可达5.12×104。 |
| 关 键 词： | 去甲氯胺酮 半抗原 人工抗原 合成 鉴定 |
Synthesis and Identification of Hapten and Complete Antigen of Norketa mine |
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| YANG Ting-Ting,ZHAO Chun-Cheng,SHEN Xiao-Ping,TAN Bo-Chang,ZOU Pan-Kun,WANG Huai- Ming,LIU Ling-Yun,WEI Chao,WEI Wan-Li,HE Jin-Hai,WU Jie,ZHANG Dong-Sheng,ZHAO Xiao-Lian.Synthesis and Identification of Hapten and Complete Antigen of Norketa mine[J].Chinese Journal of Analytical Chemistry,2010,38(1). | |
| Authors: | YANG Ting-Ting ZHAO Chun-Cheng SHEN Xiao-Ping TAN Bo-Chang ZOU Pan-Kun WANG Huai- Ming LIU Ling-Yun WEI Chao WEI Wan-Li HE Jin-Hai WU Jie ZHANG Dong-Sheng ZHAO Xiao-Lian |
| Institution: | Jiangsu SUWEI Microbiology Research Co.;LTD.;Wuxi 214063;Wuxi Municipal Public Security Bureau;Wuxi 214002;Zhenjiang Municipal public Security Bureau;Zhenjiang 212001;Jiangyin Municipal public Security Bureau;Jiangyin 214405;Yancheng Municipal public Security Bureau;Yancheng 224001 |
| Abstract: | Under low temperature conditions,the hapten carboxyl-norketamine was synthesized by reacting norketamine and succinaldehyde acid.Identification result using electrospray ionization mass showed the hapten was successfully synthesized.The artificial antigen confirmed by infrared spectroscopy was developed by conjugating hapten to carrier proteins with carbodiimide(EDC) method.Matrix-assisted laser desorption ionization time of flight mass spectrometry(MALDI-TOF-MS) showed that the ratio of hapten to BSA was 1... |
| Keywords: | Norketamine Hapten Artificial antigen Identification |
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