新型低毒AgInS2量子点的制备和表征以及对肿瘤细胞的成像

在水相中快速合成AgInS2( AIS)量子点，并对其紫外-可见吸收光谱及荧光发射光谱进行了表征，通过电感耦合等离子体发射光谱仪分析了其元素组成，采用高分辨透射电镜观察其形貌与分布，并验证了它相对于传统的CdTe量子点在光稳定性和细胞毒性方面的优势。在AgInS2表面连接了叶酸实现了对肿瘤细胞的靶向成像。

Synthesis and Characterization of AgInS2 Quantum Dots and Detection of Tumor Cells by Folic Acid-Conjugated AgInS2 Quantum Dots

The low-toxic AgInS2 QDs were synthesized in aqueous phase and their absorption and photoluminescence spectra were characterized. The low-toxic AgInS2 QDs had a broad absorption spectra and the emission peak was around 700 nm. The composition elements were analyzed via ICP-OES. High resolution transmission electron microscopy ( RTEM) was used to characterize the size and distribution of the synthesized low-toxic AgInS2 QDs. Then, the photostability and cytotoxicity of AgInS2 QDs were compared with the traditional CdTe QDs. The results showed that the AgInS2 QDs were much more stable than CdTe under the irradiation of a 405-nm laser. Without heavy metal elements, the cytotoxicity of AgInS2 QDs was much lower than CdTe QDs, which meant it was more likely to be applied in the biological field. Folic acid ( FA) was known as an effective tumor targeting molecule. When AgInS2 QDs were combined with folic acid, the FA-AgInS2 QDs could be used to distinguish tumor cells between the normal cells and cancer cells. The results showed the AgInS2 QDs possessed great application prospects in the in vivo tumor detection.