肌红蛋白的同步荧光光谱

首次对肌红蛋白的同步荧光光谱进行了研究，并对肌红蛋白荧光峰予以归属。当△λ为20nm时，308nm处的荧光峰主要为酪氨酸残基的贡献，很小一部分是由色氨酸残基贡献的；△λ为40nm时，分别在322和596nm处观察到两个荧光峰，322nm的荧光峰为酪氨酸和色氨酸残基的共同贡献，596nm的荧光峰则归属为肌红蛋白分子中血红素的贡献。

Synchronous Fluorescence Spectra of Myoglobin

The synchronous fluorescence spectra of myoglobin were studies for the first time.The fluorescence peaks observed in the spectra were assigned. When the wavelength interval ( ) is 80 nm ,the main peak at 335 nm is originated ho the tryptophan residues in the myoglobin molecule.When is 20 nm, the peak at 308 nm is mainly due to the tyrosine residues in the myoglobin molecule and in a small part due to the tryptophan residues. Two peaks at 322 and 596 nm were observed in the spectrum of myoglobin for =40 nm. The peak at 322 nm is due to both tyrosine and tryptophan residues. The peak at 596 nm is attributed to the heme group in the myoglobin molecule.