| 核磁共振和液相色谱-质谱平行动态谱学方法对连翘活性提取物的同步结构分析 |
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| 引用本文： | 刘影,贺玖明,孙瑞祥,刘超,张瑞萍,石建功,再帕尔·阿不力孜.核磁共振和液相色谱-质谱平行动态谱学方法对连翘活性提取物的同步结构分析[J].分析化学,2011,39(3):323-329. |
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| 作者姓名： | 刘影 贺玖明 孙瑞祥 刘超 张瑞萍 石建功 再帕尔·阿不力孜 |
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| 作者单位： | 中国医学科学院/北京协和医学院药物研究所中草药物质基础与资源利用教育部重点实验室;中国科学院计算技术研究所; |
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| 基金项目： | 国家自然科学基金(No.20775091)资助 |
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| 摘 要： | 以连翘植物的活性提取物为研究对象,基于不完全分离分析策略,以制备型HPLC技术为分离手段,获取含量呈连续动态变化的系列混合物,运用NMR/LC-MS 平行动态谱学方法对提取物中苯乙醇苷类成分进行了同步结构分析研究.采用本方法可获取系列提取物样品中同一成分内在相关且互补的谱学信息,包括保留时间、质荷比(m/z)和化学位移...
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| 关 键 词： | NMR/LC-MS平行动态谱 不完全分离 多组分同步结构鉴定 连翘植物 苯乙醇苷类 |
Simultaneous Structural Identification of Constituents in Active Herbal Extract of Forsythia Suspensa Using Nuclear Magnetic Resonance/Liquid Chromatography-Mass Spectrometry Parallel Dynamic Spectroscopy |
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| LIU Ying,HE Jiu-Ming,SUN Rui-Xiang,LIU Chao,ZHANG Rui-Ping,SHI Jian-Gong,Abliz ZEPER.Simultaneous Structural Identification of Constituents in Active Herbal Extract of Forsythia Suspensa Using Nuclear Magnetic Resonance/Liquid Chromatography-Mass Spectrometry Parallel Dynamic Spectroscopy[J].Chinese Journal of Analytical Chemistry,2011,39(3):323-329. |
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| Authors: | LIU Ying HE Jiu-Ming SUN Rui-Xiang LIU Chao ZHANG Rui-Ping SHI Jian-Gong Abliz ZEPER |
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| Institution: | LIU Ying1,HE Jiu-Ming1,SUN Rui-Xiang2,LIU Chao2,ZHANG Rui-Ping1,SHI Jian-Gong1,Abliz ZEPER11(Key Laboratory of Bioactive Substances and Resource Utilization of Chinese Herbal Medicine,Ministry of Education,Institute of Materia Medica,Chinese Academy of Medical Sciences andPeking Union Medical College,Beijing 100050) 2(Institute of Computing Technology,Chinese Academy of Sciences,Beijing 100080) |
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| Abstract: | Based on an incomplete separation strategy,the crude extract of Forsythia Suspensa was separated into a series of fractions with the concentration of constituent dynamic variation by RP preparative chromatography.NMR/LC-MS parallel dynamic spectroscopic(PDS) method was applied to identify the phenylethanoid glycosides in these fractions.The complementary spectral information of the same individual constituent in the crude extract was simultaneously obtained from the spectra of mixtures,including retention t... |
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| Keywords: | Nuclear magnetic resonance/liquid chromatography/mass spectrometry parallel dynamic spectroscopy Incomplete separation strategy Simultaneous structural identification in mixture Forsythia Suspensa Phenylethanoid glycosides |
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