| 辛巴蓝F-3GA修饰的啤酒废酵母菌亲和吸附溶菌酶 |
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| 引用本文： | 陈聪,孙小梅,李步海.辛巴蓝F-3GA修饰的啤酒废酵母菌亲和吸附溶菌酶[J].分析化学,2011,39(1):91-94. |
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| 作者姓名： | 陈聪 孙小梅 李步海 |
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| 作者单位： | 中南民族大学化学与材料科学学院分析化学国家民委重点实验室; |
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| 基金项目： | 国家民委院校科研基金(No.MJY00004)资助项目 |
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| 摘 要： | 利用j嗪染料辛巴蓝F-3GA修饰经戊二醛交联的啤酒废酵母菌,得到一种新型染料亲和吸附剂.辛巴蓝F-3GA的固载量为161.1 mg/g.以溶菌酶为研究对象,考察吸附时间、酶初始浓度、pH值、离子强度等因素对吸附率的影响.结果表明:当pH=7.0时,其对溶菌酶有较高的吸附量(229.1 mg/g),吸附性能明显优于未接枝...
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| 关 键 词： | 啤酒废酵母菌 辛巴蓝F-3GA 染料亲和吸附 溶菌酶 |
Adsorption of Waste Beer Yeast Modified with Cibacron Blue F-3GA for Lysozyme |
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| CHEN Cong,SUN Xiao-Mei,LI Bu-Hai.Adsorption of Waste Beer Yeast Modified with Cibacron Blue F-3GA for Lysozyme[J].Chinese Journal of Analytical Chemistry,2011,39(1):91-94. |
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| Authors: | CHEN Cong SUN Xiao-Mei LI Bu-Hai |
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| Institution: | CHEN Cong,SUN Xiao-Mei,LI Bu-Hai*(Key Laboratory of Analytical Chemistry of the State Ethnic Affairs Comission,South-central University for Nationalities,Wuhan 430074) |
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| Abstract: | The waste beer yeast was crosslinked with glutaraldehyde and modified with Cibacron B1ue F-3GA.A new dye affinity adsorbent was prepared.The amount of immobilized Cibacron Blue F-3GA on the crosslinked yeasts was 161.1 mg/g.The effects of contact time,initial lysozyme concentration,medium pH and ionic strength on the adsorption of modified yeasts for lysozyme were studied.The dye affinity adsorbents were used for lysozyme purification from chicken egg white.The lysozyme adsorption capacity of the modified y... |
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| Keywords: | Waste beer yeast Cibacron Blue F-3GA Dye affinity adsorbent Lysozyme |
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