| 毛细管等电聚焦/加压毛细管电色谱二维分离体系在多肽分离中的应用 |
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| 引用本文： | 魏娟,谷雪,王彦,阎超.毛细管等电聚焦/加压毛细管电色谱二维分离体系在多肽分离中的应用[J].分析化学,2011,39(2):188-192. |
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| 作者姓名： | 魏娟 谷雪 王彦 阎超 |
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| 作者单位： | 上海交通大学药学院,上海,200240 |
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| 基金项目： | 国家自然科学基金(No.20705018); 姑苏创新创业领军人才项目(No.ZGX0727); 国家科技部农业科技成果转化资金(No.2008002); 上海市科委科学仪器环境条件建设项目(No.09142200202)资助课题 |
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| 摘 要： | 以毛细管等电聚焦(cIEF)为第一维分离模式,以反相加压毛细管电色谱(pCEC)为第二维分离模式,开展离线二维色谱分离研究,并对复杂肽段进行分离.羟丙基纤维紊(HPC)涂层的毛细管用于cIEF分离,对6种标准蛋白质的平均分离柱效约为31万.在毛细管末端引进电隔离槽,方便了第一维样品的收集.在加电6 kV下,第二维pCE...
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| 关 键 词： | 毛细管等电聚焦 加压毛细管电色谱 多维分析 多肽 |
Two-Dimensional Separation System by Coupling Capillary Isoelectric Focusing to Pressurized Capillary Electrochro-matography for Peptides Separation |
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| WEI Juan,GU Xue,WANG Yan,YAN Chao.Two-Dimensional Separation System by Coupling Capillary Isoelectric Focusing to Pressurized Capillary Electrochro-matography for Peptides Separation[J].Chinese Journal of Analytical Chemistry,2011,39(2):188-192. |
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| Authors: | WEI Juan GU Xue WANG Yan YAN Chao |
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| Institution: | WEI Juan,GU Xue*,WANG Yan,YAN Chao*(School of Pharmacy,Shanghai Jiao Tong University,Shanghai 200240) |
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| Abstract: | A novel 2D platform combining capillary isoelectric focusing(cIEF)with pressurized capillary electrochromatography(pCEC)has been developed for off-line separation of peptides.The HPC coated capillary of cIEF was applied in the separation of standard proteins,and the average efficiency was around 310000.A homemade partition cell was induced in the end of cIEF capillary to make the collection of the eluting section more convenient.Compared to the capillary reversed phase liquid chromatography(CRPLC),the 6 kV ... |
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| Keywords: | Pressurized capillary electrochromatography Capillary isoelectric focusing Muti-dimension Peptides |
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