| 吡罗昔康与蛋白质作用特征的热力学研究 |
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| 引用本文： | 颜承农,上官云凤,潘祖亭,刘义,屈松生.吡罗昔康与蛋白质作用特征的热力学研究[J].分析化学,2004,32(3):317-319. |
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| 作者姓名： | 颜承农 上官云凤 潘祖亭 刘义 屈松生 |
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| 作者单位： | 长江大学化学系,荆州,434020;武汉大学化学与分子科学学院,武汉,430072 |
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| 基金项目： | 国家自然科学基金 (No .2 0 2 75 0 2 8)，湖北省教育厅科研重点基金 (No .2 0 0 3A0 0 3 )资助项目 |
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| 摘 要： | 用荧光光谱法和吸收光谱法研究了吡罗昔康与牛血清白蛋白结合反应，显示吡罗昔康能强烈猝灭对牛血清白蛋白的荧光强度，根据荧光猝灭数据，并由Stern-Volmer和Lineweaver-Burk方程分析并处理实验数据，得到反应的结合常数、热力学参数等。
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| 关 键 词： | 吡罗昔康 牛血清白蛋白 荧光光谱法 吸收光谱 热力学参数 |
Thermodynamics Studies on the Reaction Characteristics between Piroxican and Bovine Serum Albumin |
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| Yan Chengnong,Shangguan Yunfeng.Thermodynamics Studies on the Reaction Characteristics between Piroxican and Bovine Serum Albumin[J].Chinese Journal of Analytical Chemistry,2004,32(3):317-319. |
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| Authors: | Yan Chengnong Shangguan Yunfeng |
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| Abstract: | The binding reaction between piroxican and bovine serum albumin (BSA) was studied by using fluorescence spectra and absorption spectra. It is shown that this compound has a powerful ability to quench the fluorescence intensity of BSA, The fluorescence quenching data were analyzed according to Stern-Volmer equation and Lineweaver-Burk equation, and the binding constant, and the thermodynamic parameters were obtained. |
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| Keywords: | Piroxican bovine serum albumin fluorescence spectrum absorption spectrum thermodynamic parameters |
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