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| 细胞色素C在4,5-二氮芴-9-酮修饰玻碳电极上的直接电化学研究 | |
| 引用本文： | 尹起范,韩文霞,朱玉兰,徐继明,魏科霞,史昆波.细胞色素C在4,5-二氮芴-9-酮修饰玻碳电极上的直接电化学研究[J].分析化学,2009,37(1). |
| 作者姓名： | 尹起范 韩文霞 朱玉兰 徐继明 魏科霞 史昆波 |
| 作者单位： | 1. 淮阴师范学院化学系,江苏省低维材料化学重点建设实验室,淮安,223300;延边大学理学院化学系,延吉,133002 2. 延边大学理学院化学系,延吉,133002 3. 淮阴师范学院化学系,江苏省低维材料化学重点建设实验室,淮安,223300 |
| 基金项目： | 国家自然科学基金，江苏省教育厅自然科学基金，江苏省低维材料化学重点建设实验室开放课题 |
| 摘 要： | 采用循环伏安法探讨了细胞色素C(Cyt C)在4, 5-二氮芴-9-酮(dafo)修饰玻碳电极表面的电化学行为.结果表明,Cyt C在dafo修饰电极上呈现一对峰形较好且准可逆的氧化还原峰,其式电位(E0′)为13 mV,峰电流与扫描速度呈线性关系,该电极过程是表面控制过程,电化学反应效率常数(ks)为0.89/s.固定在dafo上的Cyt C能促进H2O2的催化还原,响应快速而灵敏(<10 s),峰电流与H2O2浓度在5.0×10-6～3.0×10-4 mol/L范围内呈线性关系;检出限2.5×10-6 mol/L;米氏常数为1.07 mmol/L,显示出较好的亲和力. |
| 关 键 词： | 细胞色素C 5-二氮芴-9-酮 修饰电极 直接电化学 |
Direct Electrochemical Behavior of Cytochrome C Immobilized on 4,5-Diazafluorene-9-one Modified Glassy Carbon Electrode |
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| YIN Qi-Fan,HAN Wen-Xia,ZHU Yu-Lan,XU Ji-Ming,WEI Ke-Xia,SHI Kun-Bo.Direct Electrochemical Behavior of Cytochrome C Immobilized on 4,5-Diazafluorene-9-one Modified Glassy Carbon Electrode[J].Chinese Journal of Analytical Chemistry,2009,37(1). | |
| Authors: | YIN Qi-Fan HAN Wen-Xia ZHU Yu-Lan XU Ji-Ming WEI Ke-Xia SHI Kun-Bo |
| Institution: | Jiangsu Key Laboratory for Chemistry of Low-Dimensional Materials;Department of Chemistry Huaiyin Teachers College;Huaian 223300;Department of Chemistry;Science College;Yanbian University;Yanji 133002 |
| Abstract: | The direct electrochemical behavior of cytochrome C(Cyt C) immobilized on 4,5-diazafluorene-9-one(dafo) modified glassy carbon electrode was investigated by cyclic voltammetry(CV).The results show that a pair of well-defined quasi-reversible redox peaks of Cyt C was obtained at Cyt C/dafo/GCE based enzyme electrode by the direct electron transfer between the protein and the electrode.The peak current was linearly dependent on the scan rate,which indicated that the direct electrochemical characteristics of C... |
| Keywords: | 4 |
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