| 纳米增效的骨唾液蛋白压电免疫传感器阵列的研制 |
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| 引用本文： | 陈钰,王捷,刘仲明.纳米增效的骨唾液蛋白压电免疫传感器阵列的研制[J].分析化学,2010,38(12). |
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| 作者姓名： | 陈钰 王捷 刘仲明 |
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| 作者单位： | 1. 广州军区广州总医院医学实验科,广州,510010;华南理工大学生物科学与工程学院,广州,510006
2. 广州军区广州总医院医学实验科,广州,510010 |
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| 基金项目： | 广东省科技计划项目(Nos.200913030801280,s008A030203005)资助 |
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| 摘 要： | 以AT切型10MHz双面镀金的石英晶体为换能器,结合纳米金颗粒(AuNPs)独特的物理化学特性,制作纳米压电免疫传感器阵列,用于临床检测血清骨唾液蛋白(BSP)。采用纳米金巯基自组装法固定抗体,通过免疫反应检测血清中BSP含量。考察了纳米金巯基自组装和巯基自组装两种抗体固定方法的固定效果,前者抗体固定量显著高于后者(P<0.05)。对稀释度为1:9～1:2的标准血清进行检测的结果显示,在该血清稀释度范围内,构建的BSP纳米压电免疫传感器阵列的准确度和灵敏度优于未使用AuNPs的传感器阵列。初步验证了乳腺癌患者和骨质疏松症患者的血清中BSP的含量均显著高于正常人(P<0.05)。使用AuNPs可以增加抗体的固定量,提高免疫反应的响应值;此传感器阵列具有结构简单、稳定性好、灵敏度高、特异性好等优点,可用于BSP的定量检测。
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| 关 键 词： | 压电免疫传感器 金纳米颗粒 增效 骨唾液蛋白 临床检测 |
Design of Au Nanoparticles Amplified Piezoelectric Immunosensor Array to Detect Bone Sialoprotein |
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| CHEN Yu,WANG Jie,LIU Zhong-Ming.Design of Au Nanoparticles Amplified Piezoelectric Immunosensor Array to Detect Bone Sialoprotein[J].Chinese Journal of Analytical Chemistry,2010,38(12). |
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| Authors: | CHEN Yu WANG Jie LIU Zhong-Ming |
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| Institution: | CHEN Yu1,2,WANG Jie 1,LIU Zhong-Ming1 1(Department of Medical Research,General Hospital of Guangzhou Command,Guangzhou 510010) 2(College of Bioscience and Bioengineering,South China University of Technology,Guangzhou 510006) |
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| Abstract: | With the unique properties of Au nanopaticles,a microarray was designed using AT-cut 10 MHz quartz crystal to detect bon sialoprotein(BSP) expression from clinical serum.Monoclonal antibody of BSP was immobilized by AuNPs/sulfosuccinimidyl-6-3'-(2-pyridyldithio)-propionamido]hexanoa(Sulfo-LC-SPDP) self-assembly.The serum BSP was detected after immunoreaction.With two different immobilizations,the frequency shift is higher via Au nanopaticles than that without Au nanopaticles.The detection of reference stan... |
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| Keywords: | Piezoelectric immunosensor Gold nanopatticles Ampilification Bone sialoprotein Clinical detection |
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