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| 液相色谱法检测水产品中亚甲蓝及其代谢物残留 | |
| 引用本文： | 杨方,范克伟,刘正才,林永辉,陈健,林雁飞,胡小钟,陈国南.液相色谱法检测水产品中亚甲蓝及其代谢物残留[J].分析化学,2009,37(4). |
| 作者姓名： | 杨方 范克伟 刘正才 林永辉 陈健 林雁飞 胡小钟 陈国南 |
| 作者单位： | 1. 福建出入境检验检疫局,福州,350001;食品安全分析与检测技术教育部重点实验室(福州大学),福州,350003 2. 福建农林大学动物科学学院,福州,350003 3. 福建出入境检验检疫局,福州,350001 4. 湖北出入境检验检疫局,武汉,430022 5. 食品安全分析与检测技术教育部重点实验室(福州大学),福州,350003 |
| 基金项目： | 国家科技支撑计划项目，福建省科技攻关重点项目 |
| 摘 要： | 建立了液相色谱同时检测水产品中亚甲基蓝及其代谢物天青A、天青B、天青C残留的方法并用于样品检测.试样中残留的药物以含离子对试剂对甲苯磺酸的乙酸铵缓冲液-乙腈提取,二氯甲烷反萃,MCAX固相萃取小柱净化,液相色谱测定.对提取、净化及仪器分离分析条件进行了探讨,对亚甲基蓝在分析过程的稳定性进行了研究.水产品中4种化合物在0.005～0.50 mg/kg范围内线性关系良好,相关系数为0 9946～0.9978.在0.005～0.5 mg/kg浓度范围内,平均加标回收率为75.9%～93.2%; 相对标准偏差为3 3%～11.9%; AZA、AZB、AZC、MB的检出限分别为1.27、0.98、1.48和1.33μg/kg.方法稳定可靠,可满足水产品中亚甲基蓝及其代谢物的检测需要. |
| 关 键 词： | 亚甲基蓝 天青 代谢物 水产品 液相色谱 |
Determination of Methylene Blue and Its Metabolites in Aquatic Products by High Performance Liquid Chromatography |
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| YANG Fang,FAN Ke-Wei,LIU Zhen-Cai,LIN Yong-Hui,CHEN Jian,LIN Yan-Fei,HU Xiao-Zhong,CHEN Guo-Nan.Determination of Methylene Blue and Its Metabolites in Aquatic Products by High Performance Liquid Chromatography[J].Chinese Journal of Analytical Chemistry,2009,37(4). | |
| Authors: | YANG Fang FAN Ke-Wei LIU Zhen-Cai LIN Yong-Hui CHEN Jian LIN Yan-Fei HU Xiao-Zhong CHEN Guo-Nan |
| Institution: | Fujian Entry-Exit Inspection and Quarantine Bureau;Fuzhou 350001;Hubei Entry-Exit Inspection and Quarantine Bureau;Wuhan 430022;Ministry of Education Key Laboratory of Analysis and Detection Technology for Food Safety andDepartment of Chemistry;Fuzhou University;Fuzhou 350003;College of Animal Science;Fujian Agriculture and Forestry University;Fuzhou 350003 |
| Abstract: | An effective high performance liquid chromatographic method has been developed for the determination of dye methylene blue(MB) and its metabolites azure A(AZA),azure B(AZB),azure C(AZC) in aquatic products,and applied to samples of incurred samples,which had been dosed with methylene blue. Based on ion-pair formation with p-toluenesulfonic acid,the analytes were extracted from homogenised tissue using acetonitrile,partitioned against methylene dichloride ,and purified with a mixed-mode cation exchange solid... |
| Keywords: | Methylene blue azure metabolites aquatic products liquid chromatography |
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