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一种简单的葡萄糖测定方法
引用本文:韦寿莲,邓光辉.一种简单的葡萄糖测定方法[J].分析化学,2001,29(4):425-427.
作者姓名:韦寿莲  邓光辉
作者单位:1. 广西民族学院化学化工系, 西江大学轻化系,
2. 广西民族学院化学化工系,
基金项目:广西自然科学基金资助项目!(No.9912001)
摘    要:报道了一种新的葡萄糖测定方法。它是基于可逆的光诱导电子转移过程,从而实现葡萄糖的快速测定。荧光染料(3-〔5-(4-二甲氨基苯基)-4,5-二氢唑-2-苯磺酰氨基〕苯硼酸)被首次应用于体液中葡萄糖的直接测定。方法的线性范围为0~1×10-2 mol/L,检测限为5×10-5 mol/L。用建立的方法对3个血清样品中葡萄糖的直接测定,结果满意,相对标准偏差小于4%。

关 键 词:葡萄糖  光诱导电子转移  荧光染料  测定方法  血清

A Simple Method for the Determination of Glucose
Deng Guanghui,Wei Shoulian.A Simple Method for the Determination of Glucose[J].Chinese Journal of Analytical Chemistry,2001,29(4):425-427.
Authors:Deng Guanghui  Wei Shoulian
Abstract:A novel method for the determination of glucose was reported. Based on a reverse photoinduced electron transfer process, we can quickly determine the concentration of glucose in the sample. A fluorescent boronic acid, dapoxylTM 3-sulfonamidophenylboronic acid was first used for the determination of glucose. This linear range is 0~1×10-2 mol/L, the detection limit is 5×10-5 mol/L. This method has been applied to direct determination of glucosein in three kinds of serum samples with satisfactory results, the RSD is less than 4%.
Keywords:Glucose  reverse photoinduced electron transfer  dapoxylTM 3-  sulfonamidophenylboronic acid  
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