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快速高分离液相色谱-串联质谱法测定植物组织中雌激素玉米赤霉醇类化合物
引用本文:张岩,李挥,张敬轩,吕品,王丽霞,刘敬泽.快速高分离液相色谱-串联质谱法测定植物组织中雌激素玉米赤霉醇类化合物[J].分析化学,2009,37(11).
作者姓名:张岩  李挥  张敬轩  吕品  王丽霞  刘敬泽
作者单位:1. 河北师范大学生命科学学院,石家庄,050016;河北省食品安全实验室,石家庄,050051
2. 河北省食品安全实验室,石家庄,050051
3. 河北医科大学基础医学院,石家庄,050017
4. 河北师范大学生命科学学院,石家庄,050016
摘    要:应用快速高分离液相色谱-串联质谱仪(RRLC-MS/MS),建立了植物组织中玉米赤霉醇类化合物(α-玉米赤霉醇、β-玉米赤霉醇、α-玉米赤霉烯醇、β-玉米赤霉烯醇、玉米赤霉酮、玉米赤霉烯酮)的检测方法.植物组织样品采用乙腈提取,碱性水溶液反萃取,经混合阴离子(MAX)固相萃取柱进行净化和富集后,用RRLC-MS/MS检测,多反应监测(MRM)模式下进行定性与定量分析.结果表明:玉米赤霉醇类化合物在0~20 μg/kg的线性范围内均具有良好的线性关系,方法检出限(LOD)为0.5 μg/kg,定量限(LOQ)为1.0 μg/kg,6种玉米赤霉醇类化合物的平均回收率为75.8%~105.4%,相对标准偏差为2.4%~12.1%.本方法可用于植物组织中玉米赤霉醇类化合物含量的测定.

关 键 词:快速高分离液相色谱-串联质谱  植物组织  玉米赤霉醇类

Determination of Zeranols in Plant Tissue by Rapid Resolution Liquid Chromatography-Mass Spectrometry/Mass Spectrometry
ZHANG Yan,LI Hui,ZHANG Jing-Xuan,L Pin,WANG Li-Xia,LIU Jing-Ze.Determination of Zeranols in Plant Tissue by Rapid Resolution Liquid Chromatography-Mass Spectrometry/Mass Spectrometry[J].Chinese Journal of Analytical Chemistry,2009,37(11).
Authors:ZHANG Yan  LI Hui  ZHANG Jing-Xuan  L Pin  WANG Li-Xia  LIU Jing-Ze
Institution:ZHANG Yan,LI Hui,ZHANG Jing-Xuan,L(U) Pin,WANG Li-Xia,LIU Jing-Ze
Abstract:A method was developed for the determination of zeranols (α,β-zearalanol,α,β-zearalenol,zear-alanone,zearalenone) with RRLC-MS/MS in the plant tissue. The samples were extracted with acetonitrile and reextracted with aqueous alkaline solution,cleaned up with MAX column and then determined by RRLC-MS/MS using multiple reaction monitoring ( MRM) scan mode. The results showed that the working curves were linear in the range of 0 -20 μg/kg. The limits of detection ( LOD) of zeranols were 0.5 μg/kg and the limits of quantification (LOQ) was 1. 0μg/kg. Extraction recoveries for zeranols ranged from 75. 8% to 105.4% and RSD was between 2.4% and 12.1%. The method is suitable for the determination of zeranols in the plant tissue.
Keywords:Rapid resolution liquid chromatography-mass spectrometry/mass spectrometry  plant tissues  zeranols  
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