源内裂解液相色谱-质谱鉴别人参皂甙Rf和拟人参皂甙F11

研究了利用源内碰撞诱导解离(in-source collision-induced d issoc iation)的高效液相色谱-大气压化学电离质谱(HPLC-APC I/MS)获取人参和西洋参的化学标志物———人参皂甙R f和拟人参皂甙F11的特征结构信息及鉴别人参和西洋参的方法。在乙腈-水梯度洗脱反相液相色谱及源内碰撞诱导解离条件下,能获得人参皂甙R f和拟人参皂甙F11的母核离子及去糖基离子的源内碰撞诱导解离谱,从其差别能清楚区分这对同分异构体。本方法对人参皂甙R f和拟人参皂甙F11的检出限能达到10-7g柱上样量,简单、快速,单次质谱实验就能鉴别人参和西洋参。

Differentiation of Ginsenoside Rf and Pseudoginsenoside F11 by In-source Collision-induced Dissociation High Performance Liquid Chromatography-Atmospheric Pressure Chemical Ionization-Mass Spectrometry

Ginsenoside Rf and pseudoginsenoside F_(11),two chemical markers of Panax ginseng and Panax quinquefolium,were analyzed using in-source collision-induced dissociation high performance liquid chromatography-atmospheric pressure chemical ionization-mass spectrometry(HPLC-APCI/MS) method.Under the gradient elution reversed-phase liquid chromatography and in-source collision-induced dissociation conditions,aglycon and deglycoslyated ions of ginsenoside Rf and pseudoginsenoside F_(11) were obviously observed.From the differences of these ions,the two isomer compounds were identified.The detection limit of ginsenoside Rf and pseudoginsenoside F_(11) is about 10~(-7)g on column.Panax ginseng and Panax quinquefolium can be distinguished in a single HPLC-APCI/MS experiment rapidly by this way.